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. Author manuscript; available in PMC: 2012 Jul 26.

Published in final edited form as: Oncogene. 2011 Jun 27;31(4):419–431. doi: 10.1038/onc.2011.246

A) V-Src transformed fibroblasts expressing luciferase were engineered to overexpress myc-tagged Trask in a tet-inducible fashion. The doxycycline-induced expression of Trask was confirmed in this anti-myc immunoblot. Arrows indicate the two forms of Trask. B) The doxycycline-induced expression of phosphorylated Trask was confirmed in this anti-phosphotyrosine immunoblot. C) 3T3v-src/Luc/TR/Trask cells were injected into the tail vein of mice being fed water with or without doxycyline (n=10) and the development of lung metastases was monitored weekly using bioluminescence imaging. D) The total luminescence over the lung fields was captured for each mouse and the quantitative results shown here according to the experimental arm. The red lines indicate the average of the values for each arm. The 8-fold difference in the average values is highly significant (2-sided t-test, p=0.04) E) Quantitative analysis of the total luminescence signal over time from the control and doxycycline-treated mice at 7, 14 and 21 days after inoculation. Error bars represent ± SEM.

A) V-Src transformed fibroblasts expressing luciferase were engineered to overexpress myc-tagged Trask in a tet-inducible fashion. The doxycycline-induced expression of Trask was confirmed in this anti-myc immunoblot. Arrows indicate the two forms of Trask. B) The doxycycline-induced expression of phosphorylated Trask was confirmed in this anti-phosphotyrosine immunoblot. C) 3T3v-src/Luc/TR/Trask cells were injected into the tail vein of mice being fed water with or without doxycyline (n=10) and the development of lung metastases was monitored weekly using bioluminescence imaging. D) The total luminescence over the lung fields was captured for each mouse and the quantitative results shown here according to the experimental arm. The red lines indicate the average of the values for each arm. The 8-fold difference in the average values is highly significant (2-sided t-test, p=0.04) E) Quantitative analysis of the total luminescence signal over time from the control and doxycycline-treated mice at 7, 14 and 21 days after inoculation. Error bars represent ± SEM.