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. 1984 Jan 25;12(2):933–943. doi: 10.1093/nar/12.2.933

Translational strategy of Solanum nodiflorum mottle virus RNA: synthesis of a coat protein precursor in vitro and in vivo.

P A Kiberstis, D Zimmern
PMCID: PMC318546  PMID: 6694913

Abstract

Solanum nodiflorum mottle virus RNA (Mr = 1.5 X 10(6)) was translated in vitro in a wheat embryo extract. Four major products were synthesized: 2 related proteins of molecular weight 100K (P100) and 67K (P67), a protein of molecular weight 38K (P38), and a methionine-lacking protein of molecular weight 28K (P28). P38 was synthesized by a minor RNA component (Mr approximately 0.4 X 10(6)) and comigrated with the only viral product detected in SNMV-infected N. clevelandii protoplasts. Antiserum raised against purified SNMV virions precipitated both in vitro- and in vivo-synthesized P38, suggesting that it is either a precursor to or an intact form of SNMV coat protein whose apparent molecular weight in purified virus preparations is 30K.

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Selected References

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