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. 1984 Jan 25;12(2):1025–1038. doi: 10.1093/nar/12.2.1025

Isolation and characterization of genomic clones corresponding to the human type II procollagen gene.

C M Strom, W B Upholt
PMCID: PMC318553  PMID: 6320112

Abstract

A recombinant human DNA library was screened using probes corresponding to the chick alpha 1 (II) procollagen gene. This resulted in the isolation of 2 different genomic clones, LgHCol(II)a and LgHCol(II)b. LgHCol(II)a was identified as corresponding to the alpha 1(II) gene by comparative hybridization and DNA sequence analysis. DNA sequence established that LgHCol(II)a extends at least from amino acid 694 of the triple helix through 54 amino acids of the COOH-propeptide. Hybridization with a probe containing only the exon at the 3' end of the chicken gene suggests that the clone contains the 3' end of the human gene. Thus LgHCol(II)a contains approximately 40% of the coding sequences of the human type II collagen gene.

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Selected References

These references are in PubMed. This may not be the complete list of references from this article.

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