Abstract
We studied the bleached mutant of Euglena gracilis Y3BUD producing carotenoids and rich in chloroplast DNA. This mutant which was produced more than twenty years ago segregates permanently, forming 30 to 50% daughter cells without carotenoids and poor in chloroplast DNA. Electron microscopy and mapping with restriction enzymes show the deletion of a complete ribosomal operon out of the three present in the original strain.
Full text
PDF









Images in this article
Selected References
These references are in PubMed. This may not be the complete list of references from this article.
- Anderson P., Roth J. Spontaneous tandem genetic duplications in Salmonella typhimurium arise by unequal recombination between rRNA (rrn) cistrons. Proc Natl Acad Sci U S A. 1981 May;78(5):3113–3117. doi: 10.1073/pnas.78.5.3113. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Ellwood M., Nomura M. Deletion of a ribosomal ribonucleic acid operon in Escherichia coli. J Bacteriol. 1980 Aug;143(2):1077–1080. doi: 10.1128/jb.143.2.1077-1080.1980. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Heizmann P., Doly J., Hussein Y., Nicolas P., Nigon V., Bernardi G. The chloroplast genome of bleached mutants of Euglena gracilis. Biochim Biophys Acta. 1981 May 29;653(3):412–415. doi: 10.1016/0005-2787(81)90197-0. [DOI] [PubMed] [Google Scholar]
- Hill C. W., Grafstrom R. H., Harnish B. W., Hillman B. S. Tandem duplications resulting from recombination between ribosomal RNA genes in Escherichia coli. J Mol Biol. 1977 Nov 5;116(3):407–428. doi: 10.1016/0022-2836(77)90077-8. [DOI] [PubMed] [Google Scholar]
- Jeffreys A. J., Flavell R. A. A physical map of the DNA regions flanking the rabbit beta-globin gene. Cell. 1977 Oct;12(2):429–439. doi: 10.1016/0092-8674(77)90119-2. [DOI] [PubMed] [Google Scholar]
- Lehner A. F., Hill C. W. Involvement of ribosomal ribonucleic acid operons in Salmonella typhimurium chromosomal rearrangements. J Bacteriol. 1980 Jul;143(1):492–498. doi: 10.1128/jb.143.1.492-498.1980. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Loughney K., Lund E., Dahlberg J. E. Deletion of an rRNA gene set in Bacillus subtilis. J Bacteriol. 1983 Apr;154(1):529–532. doi: 10.1128/jb.154.1.529-532.1983. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Manning J. E., Richards O. C. Isolation and molecular weight of circular chloroplast DNA from Euglena gracilis. Biochim Biophys Acta. 1972 Feb 15;259(3):285–296. doi: 10.1016/0005-2787(72)90304-8. [DOI] [PubMed] [Google Scholar]
- Nicolas P., Heizmann P., Nigon V. Les différents phénotypes de mutants non photosynthétiques (phot-) chez Euglena gracilis: fréquence de formation par irradiation ultraviolette. C R Seances Acad Sci III. 1982 Jan 18;294(3):145–148. [PubMed] [Google Scholar]
- Nicolas P., Innocent J. P., Nigon V. Somatic segregation and rate of greening after ultraviolet irradiation of Euglena gracilis. Mol Gen Genet. 1977 Oct 20;155(2):123–129. doi: 10.1007/BF00393150. [DOI] [PubMed] [Google Scholar]
- Stern A. I., Schiff J. A., Epstein H. T. Studies of Chloroplast Development in Euglena. V. Pigment Biosynthesis, Photosynthetic Oxygen Evolution and Carbon Dioxide Fixation during Chloroplast Development. Plant Physiol. 1964 Mar;39(2):220–226. doi: 10.1104/pp.39.2.220. [DOI] [PMC free article] [PubMed] [Google Scholar]



