Table 1.

Comparison of screens performed to identify host factors for influenza virus infection.

Authors	Virus	Target Cell	Assay(s)	Total hits	Replication?	Notes
Brass et al. [1]	A/PR/8/34	U2OS	immunostaining	254	single-cycle	Identified IFITM proteins as potent antiviral restriction factors
Hao et al. [4]	Flu-VSV-G-Renilla luciferase	DL1	luciferase assay	∼84 with obvious human homologues	single-cycle	Initial screens were performed in Drosophila cells using VSV-G psuedotyped reporter virus. Select hits were confirmed in human 293 cells with A/WSN/33 and an H5N1 isolate (A/Indonesia/7/2005).
Karlas et al. [2]	A/WSN/33	A549	immunostaining to measure primary infection, luciferase assay to titer virus yields	287	single- and multi-cycle	Many hits were confirmed using a 2009 H1N1 isolate (A/Hamburg/04/2009) and a H5N1 isolate (A/Vietnam/1204/2004).
König et al. [3]	A/WSN/33-Ren	A549	live-cell luciferase assay at 12, 24, and 36 h post-infection	298	single-cycle	HA coding sequence in the virus was replaced with the Renilla luciferase reporter gene. Many hits were confirmed with a 2009 H1N1 isolate (A/Netherland/602/2009).
Shapira et al. [5]	A/PR/8/34 (WT and ΔNS1)	HBEC	luciferase assay to titer virus yields	616	multi-cycle	Screening included analysis of yeast 2-hybrid, transcriptional profiling, IFN-β production and infection assays.