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4′-thioIDU is incorporated in virus factories. (A) Cells infected with vaccinia virus were labeled with a monoclonal antibody (red staining); (B) 4′-thioIDU incorporated in viral DNA was visualized with a monoclonal antibody and localized to virus factories (green staining); (C) a merged image shown with nuclei labeled with DAPI show that most of the compound appears to be incorporated in viral DNA rather than in host DNA in the nuclei.
