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. 1984 Feb 10;12(3):1627–1640. doi: 10.1093/nar/12.3.1627

Expression of Ty-lacZ fusions in Saccharomyces cerevisiae.

B A Bowen, A M Fulton, M F Tuite, S M Kingsman, A J Kingsman
PMCID: PMC318604  PMID: 6322112

Abstract

We have determined the nucleotide sequence of about 520 bp spanning the 5' delta regions (Figure 1) of two Tyl elements. There is an open reading frame running out of the deltas for at least 180 nucleotides into the internal region of each element. The functional significance of these open reading frames has been tested by fusing them to a defective E.coli lacZ gene. Expression of B-galactosidase in yeast transformants containing these fusions shows that Tyl elements contain functional translation signals.

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Selected References

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