Figure 5.

Protein–protein interactions of caveolar Na⁺/K⁺-ATPase detected by co-immunoprecipitation. The membrane-bound caveolar enzyme as purified in Figure 1 was solubilized by a mixture of octyl glucoside and Triton X-100 (Experimental Procedures) in which caveolin-1 retains its native oligomeric structure.^7,23 Immunoprecipitation was conducted using a polyclonal anticaveolin-1 antibody, and immunoblots were created with a monoclonal anticaveolin-1 and the other indicated antibodies.