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. 1984 Feb 24;12(4):1791–1810. doi: 10.1093/nar/12.4.1791

Fluorescent labelling of tRNA and oligodeoxynucleotides using T4 RNA ligase.

R Cosstick, L W McLaughlin, F Eckstein
PMCID: PMC318621  PMID: 6366740

Abstract

3'-O-(5'-phosphoryldeoxycytidyl) phosphorothioate and fluorescent 3'-O-(5'-phosphoryldeoxycytidyl) S-bimane phosphorothioate can be ligated to tRNA by T4 RNA ligase. They are also efficient donors for the enzymatic ligation to oligodeoxynucleotides bearing a 3'-cytidine terminus. Cytidine 3',5'-bisphosphate is also a substrate for the ligation reaction with DNA restriction fragments with a 3'-terminate cytidylic acid residue. Oligo- and polynucleotides with a 3'-phosphorothioate group react readily with electrophiles as exemplified by the reaction with monobromobimane.

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Selected References

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