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. 1984 Feb 24;12(4):1875–1887. doi: 10.1093/nar/12.4.1875

Cloning and nucleotide sequence of the simian rotavirus gene 6 that codes for the major inner capsid protein.

M K Estes, B B Mason, S Crawford, J Cohen
PMCID: PMC318627  PMID: 6322125

Abstract

The nucleotide sequence of the gene that codes for the major inner capsid protein of the simian rotavirus SA11 has been determined. A DNA copy of mRNA from gene 6 was cloned in the E. coli plasmid pBR322. The full-length gene is 1357 nucleotides long with a 5'-noncoding region of 23 nucleotides and a 3'-noncoding region of 140 nucleotides. The gene contains a single, long, open reading-frame of 1194 nucleotides capable of coding for a protein of 397 amino acids with a molecular weight of 44,816. The predicted protein product is relatively proline-rich with a net charge at neutral pH of -3.5. One stretch of 53 amino acids (encoded by nucleotides 327-485) is basic.

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Selected References

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