Skip to main content
Nucleic Acids Research logoLink to Nucleic Acids Research
. 1984 Mar 12;12(5):2421–2426. doi: 10.1093/nar/12.5.2421

Physical mapping of the ribosomal RNA genes of Mycoplasma capricolum.

G Glaser, D Amikam, S Razin
PMCID: PMC318672  PMID: 6324119

Abstract

Physical mapping of the rRNA genes of Mycoplasma capricolum was done by digestion of the mycoplasmal DNA with EcoRI, PstI and BglII and hybridization with nick-translated probes consisting of defined portions of the rrnB ribosomal RNA operon of Escherichia coli. The results indicate that the rRNA genes in the chromosome of M. capricolum are arranged in two clusters, each organized in the order 5'-16S-23S-5S-3', resembling the order of the genes in the rrnB operon, with no large spacer regions separating the genes in each cluster.

Full text

PDF
2421

Selected References

These references are in PubMed. This may not be the complete list of references from this article.

  1. Amikam D., Razin S., Glaser G. Ribosomal RNA genes in Mycoplasma. Nucleic Acids Res. 1982 Jul 24;10(14):4215–4222. doi: 10.1093/nar/10.14.4215. [DOI] [PMC free article] [PubMed] [Google Scholar]
  2. Brosius J., Ullrich A., Raker M. A., Gray A., Dull T. J., Gutell R. R., Noller H. F. Construction and fine mapping of recombinant plasmids containing the rrnB ribosomal RNA operon of E. coli. Plasmid. 1981 Jul;6(1):112–118. doi: 10.1016/0147-619x(81)90058-5. [DOI] [PubMed] [Google Scholar]
  3. Clewell D. B. Nature of Col E 1 plasmid replication in Escherichia coli in the presence of the chloramphenicol. J Bacteriol. 1972 May;110(2):667–676. doi: 10.1128/jb.110.2.667-676.1972. [DOI] [PMC free article] [PubMed] [Google Scholar]
  4. Glaser G., Enquist L., Cashel M. ColE1 cloning of a ribosomal RNA promoter region from lambdarifd18 by selection for lambda integration and excision functions. Gene. 1977;2(3-4):159–172. doi: 10.1016/0378-1119(77)90015-4. [DOI] [PubMed] [Google Scholar]
  5. Glaser G., Razin A., Razin S. Stable RNA synthesis and its control in Mycoplasma capricolum. Nucleic Acids Res. 1981 Aug 11;9(15):3641–3646. doi: 10.1093/nar/9.15.3641. [DOI] [PMC free article] [PubMed] [Google Scholar]
  6. Pollack Y., Stein R., Razin A., Cedar H. Methylation of foreign DNA sequences in eukaryotic cells. Proc Natl Acad Sci U S A. 1980 Nov;77(11):6463–6467. doi: 10.1073/pnas.77.11.6463. [DOI] [PMC free article] [PubMed] [Google Scholar]
  7. Sawada M., Osawa S., Kobayashi H., Hori H., Muto A. The number of ribosomal RNA genes in Mycoplasma capricolum. Mol Gen Genet. 1981;182(3):502–504. doi: 10.1007/BF00293942. [DOI] [PubMed] [Google Scholar]
  8. Southern E. M. Detection of specific sequences among DNA fragments separated by gel electrophoresis. J Mol Biol. 1975 Nov 5;98(3):503–517. doi: 10.1016/s0022-2836(75)80083-0. [DOI] [PubMed] [Google Scholar]
  9. Tu J., Zillig W. Organization of rRNA structural genes in the archaebacterium Thermoplasma acidophilum. Nucleic Acids Res. 1982 Nov 25;10(22):7231–7245. doi: 10.1093/nar/10.22.7231. [DOI] [PMC free article] [PubMed] [Google Scholar]
  10. Vogelstein B., Gillespie D. Preparative and analytical purification of DNA from agarose. Proc Natl Acad Sci U S A. 1979 Feb;76(2):615–619. doi: 10.1073/pnas.76.2.615. [DOI] [PMC free article] [PubMed] [Google Scholar]
  11. Weinstock R., Sweet R., Weiss M., Cedar H., Axel R. Intragenic DNA spacers interrupt the ovalbumin gene. Proc Natl Acad Sci U S A. 1978 Mar;75(3):1299–1303. doi: 10.1073/pnas.75.3.1299. [DOI] [PMC free article] [PubMed] [Google Scholar]

Articles from Nucleic Acids Research are provided here courtesy of Oxford University Press

RESOURCES