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. 1984 Apr 11;12(7):3373–3386. doi: 10.1093/nar/12.7.3373

Complete nucleotide sequence of the 23S rRNA gene of the Cyanobacterium, Anacystis nidulans.

S E Douglas, W F Doolittle
PMCID: PMC318752  PMID: 6326060

Abstract

The nucleotide sequence of the Anacystis nidulans 23S rRNA gene, including the 5'- and 3'-flanking regions has been determined. The gene is 2876 nucleotides long and shows higher primary sequence homology to the 23S rRNAs of plastids (84.5%) than to that of E. coli (79%). The predicted rRNA transcript also shares many secondary structural features with those of plastids, reinforcing the endosymbiont hypothesis for the origin of these organelles.

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Selected References

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  1. Bonen L., Doolittle W. F. On the prokaryotic nature of red algal chloroplasts. Proc Natl Acad Sci U S A. 1975 Jun;72(6):2310–2314. doi: 10.1073/pnas.72.6.2310. [DOI] [PMC free article] [PubMed] [Google Scholar]
  2. Bonen L., Doolittle W. F. Partial sequences of 16S rRNA and the phylogeny of blue-green algae and chloroplasts. Nature. 1976 Jun 24;261(5562):669–673. doi: 10.1038/261669a0. [DOI] [PubMed] [Google Scholar]
  3. Branlant C., Krol A., Sriwidada J., Brimacombe R. RNA sequences associated with proteins L1, L9, and L5, L18, L25, in ribonucleoprotein fragments isolated from the 50-S subunit of Escherichia coli ribosomes. Eur J Biochem. 1976 Nov 15;70(2):483–492. doi: 10.1111/j.1432-1033.1976.tb11039.x. [DOI] [PubMed] [Google Scholar]
  4. Brosius J., Dull T. J., Noller H. F. Complete nucleotide sequence of a 23S ribosomal RNA gene from Escherichia coli. Proc Natl Acad Sci U S A. 1980 Jan;77(1):201–204. doi: 10.1073/pnas.77.1.201. [DOI] [PMC free article] [PubMed] [Google Scholar]
  5. Doolittle W. F. Postmaturational cleavage of 23s ribosomal ribonucleic acid and its metabolic control in the blue-green alga Anacystis nidulans. J Bacteriol. 1973 Mar;113(3):1256–1263. doi: 10.1128/jb.113.3.1256-1263.1973. [DOI] [PMC free article] [PubMed] [Google Scholar]
  6. Dujon B. Sequence of the intron and flanking exons of the mitochondrial 21S rRNA gene of yeast strains having different alleles at the omega and rib-1 loci. Cell. 1980 May;20(1):185–197. doi: 10.1016/0092-8674(80)90246-9. [DOI] [PubMed] [Google Scholar]
  7. Gray J. E., Herson D. S. Functional 70S hybrid ribosomes from blue-green algae and bacteria. Arch Microbiol. 1976 Aug;109(1-2):95–99. doi: 10.1007/BF00425118. [DOI] [PubMed] [Google Scholar]
  8. Gray M. W., Doolittle W. F. Has the endosymbiont hypothesis been proven? Microbiol Rev. 1982 Mar;46(1):1–42. doi: 10.1128/mr.46.1.1-42.1982. [DOI] [PMC free article] [PubMed] [Google Scholar]
  9. Koch W., Edwards K., Kössel H. Sequencing of the 16S-23S spacer in a ribosomal RNA operon of Zea mays chloroplast DNA reveals two split tRNA genes. Cell. 1981 Jul;25(1):203–213. doi: 10.1016/0092-8674(81)90245-2. [DOI] [PubMed] [Google Scholar]
  10. Leaver C. J. Molecular integrity of chloroplast ribosomal ribonucleic acid. Biochem J. 1973 Sep;135(1):237–240. doi: 10.1042/bj1350237. [DOI] [PMC free article] [PubMed] [Google Scholar]
  11. Messing J., Crea R., Seeburg P. H. A system for shotgun DNA sequencing. Nucleic Acids Res. 1981 Jan 24;9(2):309–321. doi: 10.1093/nar/9.2.309. [DOI] [PMC free article] [PubMed] [Google Scholar]
  12. Noller H. F., Kop J., Wheaton V., Brosius J., Gutell R. R., Kopylov A. M., Dohme F., Herr W., Stahl D. A., Gupta R. Secondary structure model for 23S ribosomal RNA. Nucleic Acids Res. 1981 Nov 25;9(22):6167–6189. doi: 10.1093/nar/9.22.6167. [DOI] [PMC free article] [PubMed] [Google Scholar]
  13. Orozco E. M., Jr, Rushlow K. E., Dodd J. R., Hallick R. B. Euglena gracilis chloroplast ribosomal RNA transcription units. II. Nucleotide sequence homology between the 16 S--23 S ribosomal RNA spacer and the 16 S ribosomal RNA leader regions. J Biol Chem. 1980 Nov 25;255(22):10997–11003. [PubMed] [Google Scholar]
  14. Rochaix J. D., Darlix J. L. Composite structure of the chloroplast 23 S ribosomal RNA genes of Chlamydomonas reinhardii. Evolutionary and functional implications. J Mol Biol. 1982 Aug 15;159(3):383–395. doi: 10.1016/0022-2836(82)90290-x. [DOI] [PubMed] [Google Scholar]
  15. Rochaix J. D., Malnoe P. Anatomy of the chloroplast ribosomal DNA of Chlamydomonas reinhardii. Cell. 1978 Oct;15(2):661–670. doi: 10.1016/0092-8674(78)90034-x. [DOI] [PubMed] [Google Scholar]
  16. Surzycki S. J., Rochaix J. D. Transcriptional mapping of ribosomal RNA genes of the chloroplast and nucleus of Chlamydomonas reinhardi. J Mol Biol. 1971 Nov 28;62(1):89–109. doi: 10.1016/0022-2836(71)90133-1. [DOI] [PubMed] [Google Scholar]
  17. Takaiwa F., Sugiura M. Nucleotide sequence of the 16S - 23S spacer region in an rRNA gene cluster from tobacco chloroplast DNA. Nucleic Acids Res. 1982 Apr 24;10(8):2665–2676. doi: 10.1093/nar/10.8.2665. [DOI] [PMC free article] [PubMed] [Google Scholar]
  18. Takaiwa F., Sugiura M. The complete nucleotide sequence of a 23-S rRNA gene from tobacco chloroplasts. Eur J Biochem. 1982 May;124(1):13–19. doi: 10.1111/j.1432-1033.1982.tb05901.x. [DOI] [PubMed] [Google Scholar]
  19. Walseth T. F., Johnson R. A. The enzymatic preparation of [alpha-(32)P]nucleoside triphosphates, cyclic [32P] AMP, and cyclic [32P] GMP. Biochim Biophys Acta. 1979 Mar 28;562(1):11–31. doi: 10.1016/0005-2787(79)90122-9. [DOI] [PubMed] [Google Scholar]
  20. Williamson S. E., Doolittle W. F. Genes for tRNAIle and tRNAAla in the spacer between the 16S and 23S rRNA genes of a blue-green alga: strong homology to chloroplast tRNA genes and tRNA genes of the E. coli rrnD gene cluster. Nucleic Acids Res. 1983 Jan 11;11(1):225–235. doi: 10.1093/nar/11.1.225. [DOI] [PMC free article] [PubMed] [Google Scholar]
  21. Zablen L. B., Kissil M. S., Woese C. R., Buetow D. E. Phylogenetic origin of the chloroplast and prokaryotic nature of its ribosomal RNA. Proc Natl Acad Sci U S A. 1975 Jun;72(6):2418–2422. doi: 10.1073/pnas.72.6.2418. [DOI] [PMC free article] [PubMed] [Google Scholar]

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