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. 1984 May 11;12(9):3807–3819. doi: 10.1093/nar/12.9.3807

Molecular amplification and purification of the E. coli recC gene product.

I D Hickson, K E Atkinson, L Hutton, A E Tomkinson, P T Emmerson
PMCID: PMC318791  PMID: 6328438

Abstract

The level of recC gene expression has been analysed using Mud(bla lac) fusions to the recC promoter. The constitutive level of expression is very low and remains so even under SOS inducing conditions. The recC gene product has been amplified by harnessing the gene to the phage lambda leftward promoter in a plasmid. From cells harbouring this plasmid, RecC protein, which represented approximately 6% of the total cellular protein, was purified to electrophoretic homogeneity.

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Selected References

These references are in PubMed. This may not be the complete list of references from this article.

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