Skip to main content
NCBI home page
Nucleic Acids Research logoLink to Nucleic Acids Research
. 1984 May 25;12(10):4281–4293. doi: 10.1093/nar/12.10.4281

Analysis of male sterile mutations in the mouse using haploid stage expressed cDNA probes.

K Dudley, J Potter, M F Lyon, K R Willison
PMCID: PMC318831  PMID: 6203099

Abstract

A differential hybridization screening procedure has identified cDNAs which correspond to RNAs which are expressed in mouse testis and at lower levels in liver and spleen. The sensitivity of this procedure is such that approximately 0.5% of 1.4 X 10(4) cDNA clones are revealed as "testis specific". We have focused on ten cDNA clones which have been used to identify RNAs expressed in the haploid phase of spermatogenesis. Using Northern blots to analyse RNA isolated from the testes of mutant mice (Tfm/Y and Sxr/+) blocked at specific stages in spermatogenesis or RNA from sexually immature mice, 8 clones have been identified which correspond to RNAs expressed uniquely or at much higher levels in meiotic or post meiotic cells.

Full text

PDF
4281

Images in this article

4284Image
on p.4284
4287Image
on p.4287
4288Image
on p.4288
4289Image
on p.4289

Selected References

These references are in PubMed. This may not be the complete list of references from this article.

  1. Aviv H., Leder P. Purification of biologically active globin messenger RNA by chromatography on oligothymidylic acid-cellulose. Proc Natl Acad Sci U S A. 1972 Jun;69(6):1408–1412. doi: 10.1073/pnas.69.6.1408. [DOI] [PMC free article] [PubMed] [Google Scholar]
  2. Barth R. K., Gross K. W., Gremke L. C., Hastie N. D. Developmentally regulated mRNAs in mouse liver. Proc Natl Acad Sci U S A. 1982 Jan;79(2):500–504. doi: 10.1073/pnas.79.2.500. [DOI] [PMC free article] [PubMed] [Google Scholar]
  3. Bibb M. J., Van Etten R. A., Wright C. T., Walberg M. W., Clayton D. A. Sequence and gene organization of mouse mitochondrial DNA. Cell. 1981 Oct;26(2 Pt 2):167–180. doi: 10.1016/0092-8674(81)90300-7. [DOI] [PubMed] [Google Scholar]
  4. Cattanach B. M., Pollard C. E., Hawker S. G. Sex-reversed mice: XX and XO males. Cytogenetics. 1971;10(5):318–337. doi: 10.1159/000130151. [DOI] [PubMed] [Google Scholar]
  5. Erickson R. P. t-Alleles and the possibility of post-meiotic gene expression during mammalian spermatogenesis. Fed Proc. 1978 Sep;37(11):2517–2521. [PubMed] [Google Scholar]
  6. FAWCETT D. W., ITO S., SLAUTTERBACK D. The occurrence of intercellular bridges in groups of cells exhibiting synchronous differentiation. J Biophys Biochem Cytol. 1959 May 25;5(3):453–460. doi: 10.1083/jcb.5.3.453. [DOI] [PMC free article] [PubMed] [Google Scholar]
  7. Fujimoto H., Erickson R. P. Functional assays for mRNA detect many new messages after male meiosis in mice. Biochem Biophys Res Commun. 1982 Oct 29;108(4):1369–1375. doi: 10.1016/s0006-291x(82)80057-0. [DOI] [PubMed] [Google Scholar]
  8. Geremia R., Boitani C., Conti M., Monesi V. RNA synthesis in spermatocytes and spermatids and preservation of meiotic RNA during spermiogenesis in the mouse. Cell Differ. 1977 Mar;5(5-6):343–355. doi: 10.1016/0045-6039(77)90072-0. [DOI] [PubMed] [Google Scholar]
  9. Gold B., Fujimoto H., Kramer J. M., Erickson R. P., Hecht N. B. Haploid accumulation and translational control of phosphoglycerate kinase-2 messenger RNA during mouse spermatogenesis. Dev Biol. 1983 Aug;98(2):392–399. doi: 10.1016/0012-1606(83)90368-8. [DOI] [PubMed] [Google Scholar]
  10. Gold B., Stern L., Bradley F. M., Hecht N. B. Gene expression during mammalian spermatogenesis. II. Evidence for stage-specific differences in mRNA populations. J Exp Zool. 1983 Jan;225(1):123–134. doi: 10.1002/jez.1402250115. [DOI] [PubMed] [Google Scholar]
  11. Hammerberg C., Klein J. Evidence for postmeiotic effect of T factors causing segregation distortion in mouse. Nature. 1975 Jan 10;253(5487):137–138. doi: 10.1038/253137a0. [DOI] [PubMed] [Google Scholar]
  12. Hanahan D. Studies on transformation of Escherichia coli with plasmids. J Mol Biol. 1983 Jun 5;166(4):557–580. doi: 10.1016/s0022-2836(83)80284-8. [DOI] [PubMed] [Google Scholar]
  13. Helfman D. M., Feramisco J. R., Fiddes J. C., Thomas G. P., Hughes S. H. Identification of clones that encode chicken tropomyosin by direct immunological screening of a cDNA expression library. Proc Natl Acad Sci U S A. 1983 Jan;80(1):31–35. doi: 10.1073/pnas.80.1.31. [DOI] [PMC free article] [PubMed] [Google Scholar]
  14. Kleene K. C., Distel R. J., Hecht N. B. cDNA clones encoding cytoplasmic poly(A)+ RNAs which first appear at detectable levels in haploid phases of spermatogenesis in the mouse. Dev Biol. 1983 Aug;98(2):455–464. doi: 10.1016/0012-1606(83)90375-5. [DOI] [PubMed] [Google Scholar]
  15. Linzer D. I., Nathans D. Growth-related changes in specific mRNAs of cultured mouse cells. Proc Natl Acad Sci U S A. 1983 Jul;80(14):4271–4275. doi: 10.1073/pnas.80.14.4271. [DOI] [PMC free article] [PubMed] [Google Scholar]
  16. Lyon M. F., Hawkes S. G. X-linked gene for testicular feminization in the mouse. Nature. 1970 Sep 19;227(5264):1217–1219. doi: 10.1038/2271217a0. [DOI] [PubMed] [Google Scholar]
  17. MONESI V. RIBONUCLEIC ACID SYNTHESIS DURING MITOSIS AND MEIOSIS IN THE MOUSE TESTIS. J Cell Biol. 1964 Sep;22:521–532. doi: 10.1083/jcb.22.3.521. [DOI] [PMC free article] [PubMed] [Google Scholar]
  18. Messing J., Vieira J. A new pair of M13 vectors for selecting either DNA strand of double-digest restriction fragments. Gene. 1982 Oct;19(3):269–276. doi: 10.1016/0378-1119(82)90016-6. [DOI] [PubMed] [Google Scholar]
  19. OAKBERG E. F. A description of spermiogenesis in the mouse and its use in analysis of the cycle of the seminiferous epithelium and germ cell renewal. Am J Anat. 1956 Nov;99(3):391–413. doi: 10.1002/aja.1000990303. [DOI] [PubMed] [Google Scholar]
  20. OAKBERG E. F. Duration of spermatogenesis in the mouse and timing of stages of the cycle of the seminiferous epithelium. Am J Anat. 1956 Nov;99(3):507–516. doi: 10.1002/aja.1000990307. [DOI] [PubMed] [Google Scholar]
  21. Ohno S. Major regulatory genes for mammalian sexual development. Cell. 1976 Mar;7(3):315–321. doi: 10.1016/0092-8674(76)90160-4. [DOI] [PubMed] [Google Scholar]
  22. Rigby P. W., Dieckmann M., Rhodes C., Berg P. Labeling deoxyribonucleic acid to high specific activity in vitro by nick translation with DNA polymerase I. J Mol Biol. 1977 Jun 15;113(1):237–251. doi: 10.1016/0022-2836(77)90052-3. [DOI] [PubMed] [Google Scholar]
  23. Scott M. R., Westphal K. H., Rigby P. W. Activation of mouse genes in transformed cells. Cell. 1983 Sep;34(2):557–567. doi: 10.1016/0092-8674(83)90388-4. [DOI] [PubMed] [Google Scholar]
  24. Southern E. M. Detection of specific sequences among DNA fragments separated by gel electrophoresis. J Mol Biol. 1975 Nov 5;98(3):503–517. doi: 10.1016/s0022-2836(75)80083-0. [DOI] [PubMed] [Google Scholar]
  25. Stern L., Kleene K. C., Gold B., Hecht N. B. Gene expression during mammalian spermatogenesis. III. Changes in populations of mRNA during spermiogenesis. Exp Cell Res. 1983 Jan;143(1):247–255. doi: 10.1016/0014-4827(83)90125-8. [DOI] [PubMed] [Google Scholar]
  26. Taub F., Thompson E. B. An improved method for preparing large arrays of bacterial colonies containing plasmids for hybridization: in situ purification and stable binding of DNA on paper filters. Anal Biochem. 1982 Oct;126(1):222–230. doi: 10.1016/0003-2697(82)90133-6. [DOI] [PubMed] [Google Scholar]
  27. Thomas P. S. Hybridization of denatured RNA and small DNA fragments transferred to nitrocellulose. Proc Natl Acad Sci U S A. 1980 Sep;77(9):5201–5205. doi: 10.1073/pnas.77.9.5201. [DOI] [PMC free article] [PubMed] [Google Scholar]

Articles from Nucleic Acids Research are provided here courtesy of Oxford University Press

RESOURCES