Skip to main content
NCBI home page
Nucleic Acids Research logoLink to Nucleic Acids Research
. 1984 Jun 11;12(11):4559–4573. doi: 10.1093/nar/12.11.4559

Analysis of xanthine dehydrogenase mRNA levels in mutants affecting the expression of the rosy locus.

M Covington, D Fleenor, R B Devlin
PMCID: PMC318858  PMID: 6588363

Abstract

Xanthine dehydrogenase (XDH) mRNA levels were measured in a number of mutants and natural variants affecting XDH gene expression. Two variants, ry+4 and ry+10, contain cis-acting elements which map to a region flanking the 5' end of the XDH gene. Ry+4, which has 2-3 times more XDH protein than a wild type strain, has 3.2 times more XDH mRNA. Ry+10 has 50% of the wild type XDH level and 54% of the wild type XDH mRNA level. Three rosy mutants which map within the structural gene were also examined. Two of these had little if any XDH mRNA, but the third mutant had 1.3 times more XDH mRNA than wild type flies. Another mutant, ry2 , which contains no XDH protein and has a 9KB transposable element inserted into the XDH gene, has normal levels of XDH mRNA transcripts which are also the same size as those found in the wild type strain. Changes in XDH mRNA levels were measured during Drosophila development and found to parallel changes in the amount of XDH protein. In addition, there were no large changes in the size of XDH mRNA during development.

Full text

PDF
4559

Images in this article

4564Image
on p.4564
4566Image
on p.4566
4567Image
on p.4567
4569Image
on p.4569

Selected References

These references are in PubMed. This may not be the complete list of references from this article.

  1. Bender W., Spierer P., Hogness D. S. Chromosomal walking and jumping to isolate DNA from the Ace and rosy loci and the bithorax complex in Drosophila melanogaster. J Mol Biol. 1983 Jul 25;168(1):17–33. doi: 10.1016/s0022-2836(83)80320-9. [DOI] [PubMed] [Google Scholar]
  2. Benyajati C., Spoerel N., Haymerle H., Ashburner M. The messenger RNA for alcohol dehydrogenase in Drosophila melanogaster differs in its 5' end in different developmental stages. Cell. 1983 May;33(1):125–133. doi: 10.1016/0092-8674(83)90341-0. [DOI] [PubMed] [Google Scholar]
  3. Busslinger M., Moschonas N., Flavell R. A. Beta + thalassemia: aberrant splicing results from a single point mutation in an intron. Cell. 1981 Dec;27(2 Pt 1):289–298. doi: 10.1016/0092-8674(81)90412-8. [DOI] [PubMed] [Google Scholar]
  4. Chovnick A., Ballantyne G. H., Baillie D. L., Holm D. G. Gene conversion in higher organisms: half-tetrad analysis of recombination within the rosy cistron of Drosophila melanogaster. Genetics. 1970 Oct;66(2):315–329. doi: 10.1093/genetics/66.2.315. [DOI] [PMC free article] [PubMed] [Google Scholar]
  5. Chovnick A., Gelbart W., McCarron M., Osmond B. Organization of the rosy locus in Drosophila melanogaster: evidence for a control element adjacent to the xanthine dehydrogenase structural element. Genetics. 1976 Oct;84(2):233–255. doi: 10.1093/genetics/84.2.233. [DOI] [PMC free article] [PubMed] [Google Scholar]
  6. Chovnick A., McCarron M., Hilliker A., O'Donnell J., Gelbart W., Clark S. Gene organization in Drosophila. Cold Spring Harb Symp Quant Biol. 1978;42(Pt 2):1011–1021. doi: 10.1101/sqb.1978.042.01.101. [DOI] [PubMed] [Google Scholar]
  7. Davidson E. H., Klein W. H., Britten R. J. Sequence organization in animal DNA and a speculation on hnRNA as a coordinate regulatory transcript. Dev Biol. 1977 Jan;55(1):69–84. doi: 10.1016/0012-1606(77)90320-7. [DOI] [PubMed] [Google Scholar]
  8. Devlin R. B., Emerson C. P., Jr Coordinate accumulation of contractile protein mRNAs during myoblast differentiation. Dev Biol. 1979 Mar;69(1):202–216. doi: 10.1016/0012-1606(79)90286-0. [DOI] [PubMed] [Google Scholar]
  9. Dickinson W. J. Genetic control of enzyme expression in Drosophila: a locus influencing tissue specificity of aldehyde oxidase. J Exp Zool. 1978 Dec;206(3):333–341. doi: 10.1002/jez.1402060304. [DOI] [PubMed] [Google Scholar]
  10. Gelbart W. M., McCarron M., Pandey J., Chovnick A. Genetic limits of the xanthine dehydrogenase structural element within the rosy locus in Drosophila melanogaster. Genetics. 1974 Nov;78(3):869–886. doi: 10.1093/genetics/78.3.869. [DOI] [PMC free article] [PubMed] [Google Scholar]
  11. Gelbart W., McCarron M., Chovnick A. Extension of the limits of the XDH structural element in Drosophila melanogaster. Genetics. 1976 Oct;84(2):211–232. doi: 10.1093/genetics/84.2.211. [DOI] [PMC free article] [PubMed] [Google Scholar]
  12. Goldberg D. A. Isolation and partial characterization of the Drosophila alcohol dehydrogenase gene. Proc Natl Acad Sci U S A. 1980 Oct;77(10):5794–5798. doi: 10.1073/pnas.77.10.5794. [DOI] [PMC free article] [PubMed] [Google Scholar]
  13. Goldberg D. A., Posakony J. W., Maniatis T. Correct developmental expression of a cloned alcohol dehydrogenase gene transduced into the Drosophila germ line. Cell. 1983 Aug;34(1):59–73. doi: 10.1016/0092-8674(83)90136-8. [DOI] [PubMed] [Google Scholar]
  14. McCarron M., O'Donnell J., Chovnick A., Bhullar B. S., Hewitt J., Candido E. P. Organization of the rosy locus in Drosophila melanogaster: further evidence in support of a cis-acting control element adjacent to the xanthine dehydrogenase structural element. Genetics. 1979 Feb;91(2):275–293. doi: 10.1093/genetics/91.2.275. [DOI] [PMC free article] [PubMed] [Google Scholar]
  15. Rozek C. E., Davidson N. Drosophila has one myosin heavy-chain gene with three developmentally regulated transcripts. Cell. 1983 Jan;32(1):23–34. doi: 10.1016/0092-8674(83)90493-2. [DOI] [PubMed] [Google Scholar]
  16. Rubin G. M., Spradling A. C. Genetic transformation of Drosophila with transposable element vectors. Science. 1982 Oct 22;218(4570):348–353. doi: 10.1126/science.6289436. [DOI] [PubMed] [Google Scholar]
  17. Scholnick S. B., Morgan B. A., Hirsh J. The cloned dopa decarboxylase gene is developmentally regulated when reintegrated into the Drosophila genome. Cell. 1983 Aug;34(1):37–45. doi: 10.1016/0092-8674(83)90134-4. [DOI] [PubMed] [Google Scholar]
  18. Thomas P. S. Hybridization of denatured RNA and small DNA fragments transferred to nitrocellulose. Proc Natl Acad Sci U S A. 1980 Sep;77(9):5201–5205. doi: 10.1073/pnas.77.9.5201. [DOI] [PMC free article] [PubMed] [Google Scholar]
  19. Yen T. T., Glassman E. Electrophoretic variants of xanthine dehydrogenase in Drosophila melanogaster. Genetics. 1965 Nov;52(5):977–981. doi: 10.1093/genetics/52.5.977. [DOI] [PMC free article] [PubMed] [Google Scholar]

Articles from Nucleic Acids Research are provided here courtesy of Oxford University Press

RESOURCES