Skip to main content
NCBI home page
Nucleic Acids Research logoLink to Nucleic Acids Research
. 1984 Jun 11;12(11):4691–4701. doi: 10.1093/nar/12.11.4691

Complex allotypes of the rabbit immunoglobulin kappa light chains are encoded by structural alleles.

M A Akimenko, O Heidmann, F Rougeon
PMCID: PMC318868  PMID: 6330679

Abstract

We have isolated the rabbit immunoglobulin b9 Ck light chain gene and compared its nucleotide sequence with the b4, b4var , b5 and bas Ck sequences. In spite of the high number of substitutions found between the different rabbit Ck coding regions, only very few changes are silent. Furthermore, the nucleotide changes are clustered in segments which correlate with the bends and helical regions found in the tertiary structure of the Ck domain of the protein. The flanking regions present a higher degree of conservation than the coding regions. The two genomic EcoRI fragments hybridizing to a b4cDNA probe have been correlated with the two distinct loci, Ck1 and Ck2 : one encodes for the nominal b9 Ck allotype and the other contains the information for the bas Ck region. The b allotypes are true alleles which could have evolved by intergenic conversion.

Full text

PDF
4691

Selected References

These references are in PubMed. This may not be the complete list of references from this article.

  1. Amzel L. M., Poljak R. J. Three-dimensional structure of immunoglobulins. Annu Rev Biochem. 1979;48:961–997. doi: 10.1146/annurev.bi.48.070179.004525. [DOI] [PubMed] [Google Scholar]
  2. Beale D., Feinstein A. Structure and function of the constant regions of immunoglobulins. Q Rev Biophys. 1976 May;9(2):135–180. doi: 10.1017/s0033583500002390. [DOI] [PubMed] [Google Scholar]
  3. Benammar A., Cazenave P. A. A second rabbit kappa isotype. J Exp Med. 1982 Aug 1;156(2):585–595. doi: 10.1084/jem.156.2.585. [DOI] [PMC free article] [PubMed] [Google Scholar]
  4. Benton W. D., Davis R. W. Screening lambdagt recombinant clones by hybridization to single plaques in situ. Science. 1977 Apr 8;196(4286):180–182. doi: 10.1126/science.322279. [DOI] [PubMed] [Google Scholar]
  5. Bernstein K. E., Skurla R. M., Jr, Mage R. G. The sequences of rabbit kappa light chains of b4 and b5 allotypes differ more in their constant regions than in their 3' untranslated regions. Nucleic Acids Res. 1983 Oct 25;11(20):7205–7214. doi: 10.1093/nar/11.20.7205. [DOI] [PMC free article] [PubMed] [Google Scholar]
  6. Emorine L., Dreher K., Kindt T. J., Max E. E. Rabbit immunoglobulin kappa genes: structure of a germline b4 allotype J-C locus and evidence for several b4-related sequences in the rabbit genome. Proc Natl Acad Sci U S A. 1983 Sep;80(18):5709–5713. doi: 10.1073/pnas.80.18.5709. [DOI] [PMC free article] [PubMed] [Google Scholar]
  7. Farnsworth V., Goodfliesh R., Rodkey S., Hood L. Immunoglobulin allotypes of rabbit kappa chains: polymorphism of a control mechanism regulating closely linked duplicated genes? Proc Natl Acad Sci U S A. 1976 Apr;73(4):1293–1296. doi: 10.1073/pnas.73.4.1293. [DOI] [PMC free article] [PubMed] [Google Scholar]
  8. Grosveld F. G., Dahl H. H., de Boer E., Flavell R. A. Isolation of beta-globin-related genes from a human cosmid library. Gene. 1981 Apr;13(3):227–237. doi: 10.1016/0378-1119(81)90028-7. [DOI] [PubMed] [Google Scholar]
  9. Heidmann O., Rougeon F. Diversity in the rabbit immunoglobulin kappa chain variable regions is amplified by nucleotide deletions and insertions at the V-J junction. Cell. 1983 Oct;34(3):767–777. doi: 10.1016/0092-8674(83)90533-0. [DOI] [PubMed] [Google Scholar]
  10. Heidmann O., Rougeon F. Multiple sequences related to a constant-region kappa light chain gene in the rabbit genome. Cell. 1982 Mar;28(3):507–513. doi: 10.1016/0092-8674(82)90205-7. [DOI] [PubMed] [Google Scholar]
  11. Heidmann O., Rougeon F. Multiplicity of constant kappa light chain genes in the rabbit genome: a b4b4 homozygous rabbit contains a kappa-bas gene. EMBO J. 1983;2(3):437–441. doi: 10.1002/j.1460-2075.1983.tb01441.x. [DOI] [PMC free article] [PubMed] [Google Scholar]
  12. Hieter P. A., Max E. E., Seidman J. G., Maizel J. V., Jr, Leder P. Cloned human and mouse kappa immunoglobulin constant and J region genes conserve homology in functional segments. Cell. 1980 Nov;22(1 Pt 1):197–207. doi: 10.1016/0092-8674(80)90168-3. [DOI] [PubMed] [Google Scholar]
  13. Kelus A. S., Weiss S. Variant strain of rabbits lacking immunoglobulin kappa polypeptide chain. Nature. 1977 Jan 13;265(5590):156–158. doi: 10.1038/265156a0. [DOI] [PubMed] [Google Scholar]
  14. Loenen W. A., Brammar W. J. A bacteriophage lambda vector for cloning large DNA fragments made with several restriction enzymes. Gene. 1980 Aug;10(3):249–259. doi: 10.1016/0378-1119(80)90054-2. [DOI] [PubMed] [Google Scholar]
  15. Maxam A. M., Gilbert W. Sequencing end-labeled DNA with base-specific chemical cleavages. Methods Enzymol. 1980;65(1):499–560. doi: 10.1016/s0076-6879(80)65059-9. [DOI] [PubMed] [Google Scholar]
  16. OUDIN J. L'allotypie de certains antigènes protéidiques du sérum. C R Hebd Seances Acad Sci. 1956 May 23;242(21):2606–2608. [PubMed] [Google Scholar]
  17. OUDIN J. Réaction de précipitation spécifique entre des sérums d'animaux de même espèce. C R Hebd Seances Acad Sci. 1956 May 14;242(20):2489–2490. [PubMed] [Google Scholar]
  18. Poljak R. J., Amzel L. M., Chen B. L., Phizackerley R. P., Saul F. The three-dimensional structure of the fab' fragment of a human myeloma immunoglobulin at 2.0-angstrom resolution. Proc Natl Acad Sci U S A. 1974 Sep;71(9):3440–3444. doi: 10.1073/pnas.71.9.3440. [DOI] [PMC free article] [PubMed] [Google Scholar]
  19. Proudfoot N. J., Brownlee G. G. 3' non-coding region sequences in eukaryotic messenger RNA. Nature. 1976 Sep 16;263(5574):211–214. doi: 10.1038/263211a0. [DOI] [PubMed] [Google Scholar]
  20. Rigby P. W., Dieckmann M., Rhodes C., Berg P. Labeling deoxyribonucleic acid to high specific activity in vitro by nick translation with DNA polymerase I. J Mol Biol. 1977 Jun 15;113(1):237–251. doi: 10.1016/0022-2836(77)90052-3. [DOI] [PubMed] [Google Scholar]
  21. Seidman J. G., Leder P. The arrangement and rearrangement of antibody genes. Nature. 1978 Dec 21;276(5690):790–795. doi: 10.1038/276790a0. [DOI] [PubMed] [Google Scholar]
  22. Sogn J. A., Kindt T. J. A genetic polymorphism in the constant region of rabbit b4 kappa chains. J Exp Med. 1976 Jun 1;143(6):1475–1482. doi: 10.1084/jem.143.6.1475. [DOI] [PMC free article] [PubMed] [Google Scholar]
  23. Southern E. M. Detection of specific sequences among DNA fragments separated by gel electrophoresis. J Mol Biol. 1975 Nov 5;98(3):503–517. doi: 10.1016/s0022-2836(75)80083-0. [DOI] [PubMed] [Google Scholar]
  24. Streisinger G., Okada Y., Emrich J., Newton J., Tsugita A., Terzaghi E., Inouye M. Frameshift mutations and the genetic code. This paper is dedicated to Professor Theodosius Dobzhansky on the occasion of his 66th birthday. Cold Spring Harb Symp Quant Biol. 1966;31:77–84. doi: 10.1101/sqb.1966.031.01.014. [DOI] [PubMed] [Google Scholar]
  25. Terry W. D., Hood L. E., Steinberg A. G. Genetics of immunoglobulin kappa-chains: chemical analysis of normal human light chains of differing Inv types. Proc Natl Acad Sci U S A. 1969 May;63(1):71–77. doi: 10.1073/pnas.63.1.71. [DOI] [PMC free article] [PubMed] [Google Scholar]

Articles from Nucleic Acids Research are provided here courtesy of Oxford University Press

RESOURCES