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. Author manuscript; available in PMC: 2012 Nov 10.
Published in final edited form as: Neuroscience. 2011 Aug 22;195:70–79. doi: 10.1016/j.neuroscience.2011.08.033

Figure 1. Orexin innervation of CA1 is unaltered as a function of age.

Figure 1

A,B) Double-label immunohistochemistry reveals orexin fibers (black) spanning all layers of the hippocampus and making apparent appositional contacts with GAD67+ interneurons (brown cell bodies) in both young (A) and aged (B) rats. C) A trend toward reduced orexin innervation of GAD67+ interneurons in stratum pyramidale (SP) was observed in aged rats, while interneurons in the remaining lamina were unaffected by age. D,E) Double-label immunohistochemistry for orexin fibers (black) and CaMKII+ pyramidal cells (brown) in young (D) and aged (E) rats. F) Orexin innervation of CaMKII+ pyramidal cells was unaffected by age. n = 8 young, 7 aged (SO-stratum oriens; SP- stratum pyramidale; SR-stratum radiatum; SLM-stratum lacunosum moleculare). Scale bars = approximately 100 μm (A,B) and 10 μm (D,E).