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. 1985 Dec 20;13(24):8955–8967. doi: 10.1093/nar/13.24.8955

Interaction of DAPI with double-stranded ribonucleic acids.

G Manzini, L Xodo, M L Barcellona, F Quadrifoglio
PMCID: PMC318964  PMID: 4080554

Abstract

The interaction of DAPI with natural and synthetic double-stranded polyribonucleotides was studied with different optical and calorimetric methods. The results were similar to those obtained previously with double-stranded polydeoxynucleotides, i.e. two interaction modes, the first of which shows high affinity for AU clusters and consequent strong fluorescence enhancement. The results suggest caution in the use of DAPI as selective fluorescent staining agent for DNA in the presence of RNA. A narrow groove binding model with hydrogen bonds between DAPI and AU pairs is proposed. An intercalation mechanism can be excluded because of the non planarity of DAPI molecule.

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Selected References

These references are in PubMed. This may not be the complete list of references from this article.

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