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. Author manuscript; available in PMC: 2011 Oct 10.
Published in final edited form as: Chembiochem. 2008 Jul 2;9(10):1559–1563. doi: 10.1002/cbic.200700659

Figure 3. Western blot showing ACP/PPT activation partnerships. Top) PksA ACP activation.

Figure 3

Lanes 1. molecular mass references 2. Svp 3. PPTHexA 4. NpgA 5. apo-ACPPksA 6. apo-ACPPksA / Svp 7. apo-ACPPksA / PPTHexA 8. apo-ACPPksA / NpgA. Bottom) HexA ACP activation. Lanes 1. molecular mass references 2. Svp 3. PPTHexA 4. NpgA 5. apo-ACPHexA 6. apo-ACPHexA / Svp 7. apo-ACPHexA / PPTHexA 8. apo-ACPHexA / NpgA. The blot was treated with streptavidin-horseradish peroxidase conjugate to detect biotinylated-ACPs due to PPT activation.