Table 1 . Plasmids.
Plasmid number | Description |
---|---|
pSK691 | PYKL050c-RFP in pRS305 (LEU2) |
pSK692 | PYKL050c-CFP in pRS404 (TRP1) |
pSK693 | PYKL050c-RFP in pRS305 (LEU2), targets integration to CEN8 |
pSK694 | PYKL050c-CFP in pRS404 (TRP1), targets to CEN8 |
pSK695 | PYKL050c-CFP in pRS404, targets to THR1 |
pSK724 | PGPD1-gfp*-atg (BamHI–SalI fragment) in pMJ349 (URA3) targets to ARG4 |
pSK725 | PGPD1-gfp*-R215X (BamHI–SalI fragment) in pMJ349 targets to ARG4 |
pSK726 | PYKL050c-GFP* in pRS306 (URA3) |
pSK729 | PYKL050c-GFP* in pRS306 (URA3) targets to ARG4 |