Table 3 . Analysis of incidental crossovers.
| Gfp*+ spore class | NdeI digest (site1/site2) | No. of spore clones (% of class) | Configuration of markers is consistent with: | |
|---|---|---|---|---|
| SPO11+ | spo11-HA | |||
| Rfp− Cfp− | Cut/cut | 67/72 (93.0) | 53/53 (100.0) | Single crossover associated with GFP* conversion |
| Cut/uncut | 2/72 (2.8) | 0/53 | Noncrossover conversion of gfp*-atg with distal incidental exchange | |
| Uncut/cut | 3/72 (4.2) | 0/53 | Noncrossover conversion of gfp*-R215X with proximal incidental exchange | |
| Rfp+ Cfp+ | Uncut/uncut | 2/2 (100.0) | 8/9 (88.9) | Single crossover associated with GFP* conversion |
| Cut/uncut | 0/2 | 1/9 (11.1) | Noncrossover conversion of gfp*-atg with proximal incidental exchange | |
| Rfp+ Cfp− | Uncut/cut | 43/46 (93.5) | 42/45 (93.3) | Noncrossover conversion of gfp*-R215X |
| Cut/cut | 3/46 (6.5) | 3/45 (6.7) | Crossover associated with GFP* conversion with proximal incidental exchange | |
| Rfp− Cfp+ | Cut/uncut | 9/9 (100.0) | 20/23 (87.0) | Noncrossover conversion of gfp*-atg |
| Cut/cut | 0/9 | 2/23 (8.7) | Crossover associated with GFP* conversion with distal incidental exchange | |
| Uncut/uncut | 0/9 | 1/23 (4.3) | Crossover associated with GFP* conversion with proximal incidental exchange | |
Single Gfp*+ spores of the four possible flanking marker configurations were sorted, allowed to grow, and then genotyped for the NdeI sites diagrammed in Figure 5A. Data were pooled from two independent cultures (≥39 spores analyzed per culture). The agreement with expected marker patterns is described for each class.