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. 1981 Jan;78(1):41–45. doi: 10.1073/pnas.78.1.41

Detection of a common feature in several human tumor cell lines--a 53,000-dalton protein.

L V Crawford, D C Pim, E G Gurney, P Goodfellow, J Taylor-Papadimitriou
PMCID: PMC318985  PMID: 6264441

Abstract

Human cell lines, whether derived from spontaneous tumors or transformed in vitro with simian virus 40, were found to contain a 53,000-dalton phosphoprotein (pp53) in contrast to normal human cells in which this protein was not detected. Isoelectric focusing showed that pp53 comprised several species in both simian virus 40-transformed and tumor cells. Comparison of the pp53 species from the various cell lines by partial proteolysis showed that they were similar but not identical. Among the 13 tumor cell lines examined, only 1 line, HeLa, did not contain detectable pp53. All the other tumor cell lines contained pp53, and it is suggested that this protein may be associated with their transformed state.

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Selected References

These references are in PubMed. This may not be the complete list of references from this article.

  1. AUERSPERG N. LONG-TERM CULTIVATION OF HYPODIPLOID HUMAN TUMOR CELLS. J Natl Cancer Inst. 1964 Jan;32:135–163. [PubMed] [Google Scholar]
  2. Barnstable C. J., Bodmer W. F., Brown G., Galfre G., Milstein C., Williams A. F., Ziegler A. Production of monoclonal antibodies to group A erythrocytes, HLA and other human cell surface antigens-new tools for genetic analysis. Cell. 1978 May;14(1):9–20. doi: 10.1016/0092-8674(78)90296-9. [DOI] [PubMed] [Google Scholar]
  3. Cleveland D. W., Fischer S. G., Kirschner M. W., Laemmli U. K. Peptide mapping by limited proteolysis in sodium dodecyl sulfate and analysis by gel electrophoresis. J Biol Chem. 1977 Feb 10;252(3):1102–1106. [PubMed] [Google Scholar]
  4. Crawford L. V., Lane D. P., Denhardt D. T., Harlow E. E., Nicklin P. M., Osborn K., Pim D. C. Characterization of the complex between SV40 large T antigen and the 53K host protein in transformed mouse cells. Cold Spring Harb Symp Quant Biol. 1980;44(Pt 1):179–187. doi: 10.1101/sqb.1980.044.01.021. [DOI] [PubMed] [Google Scholar]
  5. Crawford L. V., O'Farrell P. Z. Effect of alkylation on the physical properties of simian virus 40 T-antigen species. J Virol. 1979 Feb;29(2):587–596. doi: 10.1128/jvi.29.2.587-596.1979. [DOI] [PMC free article] [PubMed] [Google Scholar]
  6. Crawford L. V., Pim D. C., Lane D. P. An immunochemical investigation of SV40 T-antigens. 2. Quantitation of antigens and antibody activities. Virology. 1980 Jan 30;100(2):314–325. doi: 10.1016/0042-6822(80)90522-x. [DOI] [PubMed] [Google Scholar]
  7. DeLeo A. B., Jay G., Appella E., Dubois G. C., Law L. W., Old L. J. Detection of a transformation-related antigen in chemically induced sarcomas and other transformed cells of the mouse. Proc Natl Acad Sci U S A. 1979 May;76(5):2420–2424. doi: 10.1073/pnas.76.5.2420. [DOI] [PMC free article] [PubMed] [Google Scholar]
  8. Goodfellow P., Barnstable C., Jones E., Bodmer W. F., Crumpton M. J., Snary D. Production of specific antisera to human B lymphocytes. Tissue Antigens. 1976 Feb;7(2):105–117. doi: 10.1111/j.1399-0039.1976.tb01039.x. [DOI] [PubMed] [Google Scholar]
  9. Gurney E. G., Harrison R. O., Fenno J. Monoclonal antibodies against simian virus 40 T antigens: evidence for distinct sublcasses of large T antigen and for similarities among nonviral T antigens. J Virol. 1980 Jun;34(3):752–763. doi: 10.1128/jvi.34.3.752-763.1980. [DOI] [PMC free article] [PubMed] [Google Scholar]
  10. Hackett A. J., Smith H. S., Springer E. L., Owens R. B., Nelson-Rees W. A., Riggs J. L., Gardner M. B. Two syngeneic cell lines from human breast tissue: the aneuploid mammary epithelial (Hs578T) and the diploid myoepithelial (Hs578Bst) cell lines. J Natl Cancer Inst. 1977 Jun;58(6):1795–1806. doi: 10.1093/jnci/58.6.1795. [DOI] [PubMed] [Google Scholar]
  11. Kessler S. W. Rapid isolation of antigens from cells with a staphylococcal protein A-antibody adsorbent: parameters of the interaction of antibody-antigen complexes with protein A. J Immunol. 1975 Dec;115(6):1617–1624. [PubMed] [Google Scholar]
  12. Keydar I., Chen L., Karby S., Weiss F. R., Delarea J., Radu M., Chaitcik S., Brenner H. J. Establishment and characterization of a cell line of human breast carcinoma origin. Eur J Cancer. 1979 May;15(5):659–670. doi: 10.1016/0014-2964(79)90139-7. [DOI] [PubMed] [Google Scholar]
  13. Klein E., Klein G., Nadkarni J. S., Nadkarni J. J., Wigzell H., Clifford P. Surgace IgM specificity on cells derived from a Burkitt's lymphoma. Lancet. 1967 Nov 18;2(7525):1068–1070. doi: 10.1016/s0140-6736(67)90340-6. [DOI] [PubMed] [Google Scholar]
  14. Klein G., Luka J., Zeuthen J. Transformation induced by Epstein-Barr virus and the role of the nuclear antigen. Cold Spring Harb Symp Quant Biol. 1980;44(Pt 1):253–261. doi: 10.1101/sqb.1980.044.01.029. [DOI] [PubMed] [Google Scholar]
  15. LASFARGUES E. Y., OZZELLO L. Cultivation of human breast carcinomas. J Natl Cancer Inst. 1958 Dec;21(6):1131–1147. [PubMed] [Google Scholar]
  16. Lane D. P., Crawford L. V. T antigen is bound to a host protein in SV40-transformed cells. Nature. 1979 Mar 15;278(5701):261–263. doi: 10.1038/278261a0. [DOI] [PubMed] [Google Scholar]
  17. Lane D. P., Crawford L. V. The complex between simian virus 40 T antigen and a specific host protein. Proc R Soc Lond B Biol Sci. 1980 Nov 19;210(1180):451–463. doi: 10.1098/rspb.1980.0146. [DOI] [PubMed] [Google Scholar]
  18. Lane D. P., Robbins A. K. An immunochemical investigation of SV40 T antigens. 1. Production properties and specificity of rabbit antibody to purified simian virus 40 large-T antigen. Virology. 1978 Jun 1;87(1):182–193. doi: 10.1016/0042-6822(78)90170-8. [DOI] [PubMed] [Google Scholar]
  19. Linzer D. I., Levine A. J. Characterization of a 54K dalton cellular SV40 tumor antigen present in SV40-transformed cells and uninfected embryonal carcinoma cells. Cell. 1979 May;17(1):43–52. doi: 10.1016/0092-8674(79)90293-9. [DOI] [PubMed] [Google Scholar]
  20. Livingston D. M., Henderson I. C., Hudson J. SV40 T antigen: partial purification and properties. Cold Spring Harb Symp Quant Biol. 1975;39(Pt 1):283–289. doi: 10.1101/sqb.1974.039.01.037. [DOI] [PubMed] [Google Scholar]
  21. McCormick F., Harlow E. Association of a murine 53,000-dalton phosphoprotein with simian virus 40 large-T antigen in transformed cells. J Virol. 1980 Apr;34(1):213–224. doi: 10.1128/jvi.34.1.213-224.1980. [DOI] [PMC free article] [PubMed] [Google Scholar]
  22. Migeon B. R., Norum R. A., Corsaro C. M. Isolation and analysis of somatic hybrids derived from two human diploid cells. Proc Natl Acad Sci U S A. 1974 Mar;71(3):937–941. doi: 10.1073/pnas.71.3.937. [DOI] [PMC free article] [PubMed] [Google Scholar]
  23. Minowada J., Tsubota T., Greaves M. F., Walters T. R. A non-T, non-B human leukemia cell line (NALM-1): establishment of the cell line and presence of leukemia-associated antigens. J Natl Cancer Inst. 1977 Jul;59(1):83–87. doi: 10.1093/jnci/59.1.83. [DOI] [PubMed] [Google Scholar]
  24. O'Farrell P. Z., Goodman H. M., O'Farrell P. H. High resolution two-dimensional electrophoresis of basic as well as acidic proteins. Cell. 1977 Dec;12(4):1133–1141. doi: 10.1016/0092-8674(77)90176-3. [DOI] [PubMed] [Google Scholar]
  25. Pattillo R. A., Gey G. O. The establishment of a cell line of human hormone-synthesizing trophoblastic cells in vitro. Cancer Res. 1968 Jul;28(7):1231–1236. [PubMed] [Google Scholar]
  26. Snary D., Goodfellow P., Hayman M. J., Bodmer W. F., Crumpton M. J. Subcellular separation and molecular nature of human histocompatibility antigens (HL-A). Nature. 1974 Feb 15;247(5441):457–461. doi: 10.1038/247457a0. [DOI] [PubMed] [Google Scholar]
  27. Taylor-Papadimitriou J., Shearer M., Stoker M. G. Growth requirements of human mammary epithelial cells in culture. Int J Cancer. 1977 Dec 15;20(6):903–908. doi: 10.1002/ijc.2910200613. [DOI] [PubMed] [Google Scholar]
  28. Todaro G. J., Green H., Swift M. R. Susceptibility of human diploid fibroblast strains to transformation by SV40 virus. Science. 1966 Sep 9;153(3741):1252–1254. doi: 10.1126/science.153.3741.1252. [DOI] [PubMed] [Google Scholar]

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