Isolation of a Klebsiella pneumoniae Isolate of Sequence Type 258 Producing KPC-2 Carbapenemase in Korea

Abstract

Carbapenem-resistant Klebsiella pneumoniae isolates producing K. pneumoniae carbapenemases (KPC) were first reported in the USA in 2001, and since then, this infection has been reported in Europe, Israel, South America, and China. In Korea, the first KPC-2-producing K. pneumoniae sequence type (ST) 11 strain was detected in 2010. We report the case of a patient with a urinary tract infection caused by KPC-2-producing K. pneumoniae. This is the second report of a KPC-2-producing K. pneumoniae infection in Korea, but the multilocus sequence type was ST258. The KPC-2-producing isolate was resistant to all tested β-lactams (including imipenem and meropenem), amikacin, tobramycin, ciprofloxacin, levofloxacin, and trimethoprim-sulfamethoxazole, but was susceptible to gentamicin, colistin, polymyxin B, and tigecycline. The KPC-2-producing isolate was negative to phenotypic extended-spectrum β-lactamase (ESBL) and AmpC detection tests and positive to modified Hodge test and carbapenemase inhibition test with aminophenylboronic acid.

INTRODUCTION

The resistance of Klebsiella pneumoniae to carbapenems is mainly associated with acquired carbapenemases [1]. These carbapenemases can be Ambler class A (KPC, GES), class B (VIM, IMP), and class D (OXA-48) enzymes [2]. The most common class A carbapenemases in K. pneumoniae are the K. pneumoniae carbapenemases (KPCs) [3]. KPC-producing K. pneumoniae strains were first reported in 2001 in the USA [4] and dissemination has been reported in Europe, Israel, South America, and China [5-9]. The first KPC-2-producing K. pneumoniae strain in Korea was isolated from bronchial aspirates from a patient admitted to the intensive care unit (ICU) in 2010 [10]. KPC-producing K. pneumoniae also produce VIM or CTX-M, making it difficult to select appropriate antibiotics [11]. In addition, the mortality rate is significantly higher for patients with KPC-producing isolates than those with imipenem susceptible isolates [12]. In this report, we describe a case of infection with a KPC-2-producing K. pneumoniae isolate, sequence type (ST) 258 in Korea and various phenotypic methods for screening and confirmation.

CASE REPORT

A 70-year-old woman was admitted to the Plastic Surgery (PS) department on October 5, 2010, with a 24-h history of fever and dizziness. She had a known history of unstable angina and diabetes mellitus (2001). In November 2009, she was admitted to the PS department for a skin flap operation (February, 2010) to treat a third-degree burn to the sacral area. Two months ago, a sore, approximately 10×10 cm in size, developed at the sacral area and progressed to osteomyelitis in the sacral bone. She had no recent travel history abroad. At admission, she was pale and febrile with a temperature of 38.2℃. An intermittent fever of 37.3-38.1℃ lasted until hospital day (HD) 5. Her blood pressure was 110/80 mmHg, her pulse was 78/min, and her respiratory rate was 20/min. A laboratory investigation at the time of admission revealed a peripheral white blood cell (WBC) count of 8,730/µL (73.5% neutrophils), a hemoglobin level of 8.6 g/dL, and a platelet count of 261,000/µL. Routine blood chemistry results were AST/ALT of 7/11 U/L, alkaline phosphatase of 77 U/L, blood urea nitrogen/creatinine of 23.4/1.42 mg/dL, and total protein/albumin of 5.6/2.9 g/dL. Erythrocyte sedimentation rate and C-reactive protein were both increased to 53 mm/hr and 220.03 mg/L, respectively. The urine was yellow and turbid and routine urinalysis revealed a positive WBC (3+), and positive protein (1+). Microscopic examination of urine revealed >60 WBCs and yeast organisms in a high power field. A chest radiograph showed right pleural thickening and a little collapse of the right lower lung. An abdomen and pelvic computed tomography showed signs of cystitis and fluid collection in both the abdomen and pleural cavity.

Two sets of blood culture bottles and a urine sample were taken for microbiologic study. Aerobic and anaerobic blood cultures were all negative after 5 days of incubation. In the urine culture, Candida albicans (8×10⁴ CFU/mL) grew on a blood agar plate. On HD 32, the patient had a fever of 38.1℃. Two sets of blood culture bottles and a urine sample were collected again for culture study. The blood culture results were negative. Multidrug-resistant K. pneumoniae (KPN 1010, >10⁵ CFU/mL) was isolated from the urine. Vitek2 GN and AST-N044 (bioMérieux, Marcy l'Étoile, France) were used for species identification and antimicrobial susceptibility test, respectively. With the exception of gentamicin, all susceptibility results showed high-level minimum inhibitory concentration (MIC) values. MICs were also assessed by E-test (bioMérieux). Most antibiotics were resistant and consistent with the MIC of Vitek2. However, MICs of tigecycline and colistin were 1 and 0.25 µg/mL, respectively (Table 1). The modified Hodge test [13] demonstrated strong positivity (Fig. 1A) but AmpC and ESBL phenotypic tests [14] were negative. Carbapenemase inhibition tests were performed for discrimination of carbapenemases. Briefly, meropenem disks (Becton-Dickinson, Cockeysville, MD, USA) were supplemented with 10 µL of 4 different β-lactamase inhibitors: 60 mg/mL aminophenylboronic acid (APB; Sigma St. Louis, MO, USA), 75 mg/mL cloxacillin (Sigma), 100 mg/mL dipicolinic acid (DPA; Sigma), and 0.2 M ethylenediaminetetraacetic acid (EDTA; Sigma). A 0.5 McFarland inoculum was prepared and spread on Mueller-Hinton agar plates (Becton-Dickinson). Five disks were placed on each plate: meropenem, meropenem+APB, meropenem+cloxacillin, meropenem+DPA, meropenem+EDTA. A positive response was achieved when there was a greater than 5 mm increase of the inhibition zone diameter around disks containing β-lactamase inhibitors, as compared with the meropenem disk alone [15]. The positive result was seen only with APB (Fig. 1B).

Table 1.

MICs (µg/mL) of the KPC-2-producing Klebsiella pneumoniae isolate

Abbreviations: MIC, minimum inhibitory concentration; KPC, K. pneumoniae carbapenemase.

Fig. 1.

Results obtained with a Modified Hodge test (A) and carbapenemase inhibition test (B) for carbapenem-resistant Klebsiella pneumoniae isolate (KPN 1010).

Abbreviations: MEM, meropenem; APB, aminophenylboronic acid; CLX, cloxacillin; EDTA, ethylenediaminetetraacetic acid; DPA, dipicolinic acid.

PCR and DNA sequencing were performed with primers specific for the bla_VIM, bla_IMP, bla_SIM, bla_NDM, and bla_KPC genes [16-18]. We found only the bla_KPC-2 gene. Multilocus sequence typing (MLST) with 7 housekeeping genes (rpoB, gapA, mdh, pgi, phoE, infB, and tonB) was performed [19]. The MLST showed that the isolate belonged to the epidemic clone ST258. Initially, the patient was treated with ertapenem (1 g once a day). After multidrug-resistant (MDR) K. pneumoniae was isolated from urine and the sacral sore wound, tigecycline (50 mg twice a day) was administrated for 14 days. However, neither pyuria nor the wound subsided, and MDR K. pneumoniae was repeatedly isolated. Lastly, the patient was treated with colistin (150 mg once a day), but clinical improvement was not observed and kidney function was declining. She was managed in ICU, but expired on the 90th HD due to septic shock and multiorgan failure.

DISCUSSION

The most common mechanism of carbapenem-resistant K. pneumoniae in Korea is ESBL and/or AmpC β-lactamase plus porin loss [20]. Only a single case of KPC-2-producing strain has been described in Korea and the MLST type was ST11 [10]. This isolate showed an MDR pattern to various antibiotics, including colistin. The previous patient was treated with colistin, but the prognosis was also poor. This is the second report of a KPC-2-producing K. pneumoniae in Korea. The isolate was not associated with travel and the MLST type was ST258. ST258 accounts for 70% of KPC-producing K. pneumoniae in the USA [21]. Isolates of ST258 have also been identified in Europe and Israel, indicating an international spread of ST258 among KPC-producing K. pneumoniae [6, 7].

Most KPC-producing K. pneumoniae have been associated with other β-lactamase genes, such as the widespread ESBL gene bla_CTX-M [22]. The previous KPC-2-producing K. pneumoniae isolate in Korea also contained bla_CTX-M-15 [10]. However, the KPN 1010 isolate contained neither ESBL nor AmpC gene in this study.

The KPN 1010 isolate was susceptible to gentamicin, colistin, polymyxin B, and tigecycline, but the previous Korean isolate was nonsusceptible to gentamicin, colistin, polymyxin B, and tigecycline [10].

A modified Hodge test accurately detects KPC, but is not able to discriminate from other carbapenemases [23]. A carbapenemase inhibition test, comprising a meropenem disk, and meropenem disks supplemented with APB (for detection of class A carbapenemases), cloxacillin (for detection of AmpC β-lactamases plus porin loss), DPA or EDTA (for detection of class B metallo-carbapenemases) accurately distinguishes between several different mechanisms mediating reduced susceptibility to carbapenems in Enterobacteriaceae [15]. The isolate was positive to APB and negative to cloxacillin, DPA, and EDTA, suggesting a class A carbapenemase producing isolate.