Skip to main content
PMC home page
Proceedings of the National Academy of Sciences of the United States of America logoLink to Proceedings of the National Academy of Sciences of the United States of America
. 1981 Jan;78(1):147–151. doi: 10.1073/pnas.78.1.147

Location, function, and nucleotide sequence of a promoter for bacteriophage T3 RNA polymerase.

S Adhya, S Basu, P Sarkar, U Maitra
PMCID: PMC319008  PMID: 6264429

Abstract

The major promoters for bacteriophage T3 RNA polymerase on the T3 genome have been mapped by DNA.RNA filter hybridization. One promoter is located in a 300-base-pair Hpa I restriction fragment near the genetic "left" end of T3 DNA. The sequence in the vicinity of the major initiation site of transcription in this region has been determined. A part of the (-)strand sequence is 5' T-A-T-T-T-A-C-C-C-T-C-A-C-T-A-A-A-G-+1 G-G-A-A-U 3'. Comparison of this sequence with the prototype 23-base-pair promoter sequence for bacteriophage T7 RNA polymerase shows a striking pattern of homology and divergence. Between positions -9 and +4, the sequences are virtually identical, whereas between positions -17 and -10, the sequences are quite different. It is postulated that these sequence subsets may perform different functions in transcription initiation by the phage RNA polymerases.

Full text

PDF
147

Images in this article

148Image
on p.148
148Image
on p.148
149Image
on p.149
149Image
on p.149
150Image
on p.150

Selected References

These references are in PubMed. This may not be the complete list of references from this article.

  1. Bailey J. N., Dembinski D. R., McAllister W. T. Derivation of a restriction map of bacteriophage T3 DNA and comparison with the map of bacteriophage T7 DNA. J Virol. 1980 Jul;35(1):176–183. doi: 10.1128/jvi.35.1.176-183.1980. [DOI] [PMC free article] [PubMed] [Google Scholar]
  2. Chakraborty P. R., Bandyopadhyay P., Huang H. H., Maitra U. Fidelity of in vitro transcription of T3 deoxyribonucleic acid by bacteriophage T3-induced ribonucleic acid polymerase and by Escherichia coli ribonucleic acid polymerase. J Biol Chem. 1974 Nov 10;249(21):6901–6909. [PubMed] [Google Scholar]
  3. Chakraborty P. R., Sarkar P., Huang H. H., Maitra U. Studies on T3-induced ribonucleic acid polymerase. 3. Purification and characterization of the T3-induced ribonucleic acid polymerase from bacteriophage T3-infected Escherichia coli cells. J Biol Chem. 1973 Oct 10;248(19):6637–6646. [PubMed] [Google Scholar]
  4. Donis-Keller H., Maxam A. M., Gilbert W. Mapping adenines, guanines, and pyrimidines in RNA. Nucleic Acids Res. 1977 Aug;4(8):2527–2538. doi: 10.1093/nar/4.8.2527. [DOI] [PMC free article] [PubMed] [Google Scholar]
  5. Dunn J. J., Bautz F. A., Bautz E. K. Different template specificities of phage T3 and T7 RNA polymerases. Nat New Biol. 1971 Mar 17;230(11):94–96. doi: 10.1038/newbio230094a0. [DOI] [PubMed] [Google Scholar]
  6. Dunn J. J., McAllister W. T., Bautz E. K. In vitro transcription of T3 DNA by Escherichia coli and T3 polymerases. Virology. 1972 Apr;48(1):112–125. doi: 10.1016/0042-6822(72)90119-5. [DOI] [PubMed] [Google Scholar]
  7. Dunn J. J., McAllister W. T., Bautz E. K. Transcription in vitro of T3 DNA by Escherichia coli and T3 RNA polymerases. Analysis of the products in cell-free protein-synthesizing system. Eur J Biochem. 1972 Sep 25;29(3):500–508. doi: 10.1111/j.1432-1033.1972.tb02014.x. [DOI] [PubMed] [Google Scholar]
  8. Maitra U., Huang H. H. Initiation, release, and reinitiation of RNA chains by bacteriophage-T3-induced polymerase from T3 DNA templates (E. coli-guanosine triphosphate terminus-purified polymerase). Proc Natl Acad Sci U S A. 1972 Jan;69(1):55–59. doi: 10.1073/pnas.69.1.55. [DOI] [PMC free article] [PubMed] [Google Scholar]
  9. Maitra U. Induction of a new RNA polymerase in Escherichia coli infected with bacteriophage T3. Biochem Biophys Res Commun. 1971 Apr 16;43(2):443–450. doi: 10.1016/0006-291x(71)90773-x. [DOI] [PubMed] [Google Scholar]
  10. Maitra U., Jelinek W., Yudelevich A., Majumder H. K., Guha A. Isolation and sequence determination of 5'-terminal oligonucleotide fragments of RNA transcripts synthesized by bacteriophage T3-induced RNA polymerase from T3 DNA. Proc Natl Acad Sci U S A. 1980 Jul;77(7):3908–3911. doi: 10.1073/pnas.77.7.3908. [DOI] [PMC free article] [PubMed] [Google Scholar]
  11. Maxam A. M., Gilbert W. A new method for sequencing DNA. Proc Natl Acad Sci U S A. 1977 Feb;74(2):560–564. doi: 10.1073/pnas.74.2.560. [DOI] [PMC free article] [PubMed] [Google Scholar]
  12. McDonell M. W., Simon M. N., Studier F. W. Analysis of restriction fragments of T7 DNA and determination of molecular weights by electrophoresis in neutral and alkaline gels. J Mol Biol. 1977 Feb 15;110(1):119–146. doi: 10.1016/s0022-2836(77)80102-2. [DOI] [PubMed] [Google Scholar]
  13. Oakley J. L., Strothkamp R. E., Sarris A. H., Coleman J. E. T7 RNA polymerase: promoter structure and polymerase binding. Biochemistry. 1979 Feb 6;18(3):528–537. doi: 10.1021/bi00570a023. [DOI] [PubMed] [Google Scholar]
  14. Panayotatos N., Wells R. D. Recognition and initiation site for four late promoters of phage T7 is a 22-base pair DNA sequence. Nature. 1979 Jul 5;280(5717):35–39. doi: 10.1038/280035a0. [DOI] [PubMed] [Google Scholar]
  15. Pribnow D. Bacteriophage T7 early promoters: nucleotide sequences of two RNA polymerase binding sites. J Mol Biol. 1975 Dec 15;99(3):419–443. doi: 10.1016/s0022-2836(75)80136-7. [DOI] [PubMed] [Google Scholar]
  16. Pribnow D. Nucleotide sequence of an RNA polymerase binding site at an early T7 promoter. Proc Natl Acad Sci U S A. 1975 Mar;72(3):784–788. doi: 10.1073/pnas.72.3.784. [DOI] [PMC free article] [PubMed] [Google Scholar]
  17. Rosa M. D. Four T7 RNA polymerase promoters contain an identical 23 bp sequence. Cell. 1979 Apr;16(4):815–825. doi: 10.1016/0092-8674(79)90097-7. [DOI] [PubMed] [Google Scholar]
  18. Seeburg P. H., Nüsslein C., Schaller H. Interaction of RNA polymerase with promoters from bacteriophage fd. Eur J Biochem. 1977 Mar 15;74(1):107–113. doi: 10.1111/j.1432-1033.1977.tb11372.x. [DOI] [PubMed] [Google Scholar]
  19. Siebenlist U., Simpson R. B., Gilbert W. E. coli RNA polymerase interacts homologously with two different promoters. Cell. 1980 Jun;20(2):269–281. doi: 10.1016/0092-8674(80)90613-3. [DOI] [PubMed] [Google Scholar]
  20. Smith H. O., Birnstiel M. L. A simple method for DNA restriction site mapping. Nucleic Acids Res. 1976 Sep;3(9):2387–2398. doi: 10.1093/nar/3.9.2387. [DOI] [PMC free article] [PubMed] [Google Scholar]
  21. Southern E. M. Detection of specific sequences among DNA fragments separated by gel electrophoresis. J Mol Biol. 1975 Nov 5;98(3):503–517. doi: 10.1016/s0022-2836(75)80083-0. [DOI] [PubMed] [Google Scholar]
  22. Studier F. W., Movva N. R. SAMase gene of bacteriophage T3 is responsible for overcoming host restriction. J Virol. 1976 Jul;19(1):136–145. doi: 10.1128/jvi.19.1.136-145.1976. [DOI] [PMC free article] [PubMed] [Google Scholar]
  23. Takanami M., Sugimoto K., Sugisaki H., Okamoto T. Sequence of promoter for coat protein gene of bacteriophage fd. Nature. 1976 Mar 25;260(5549):297–302. doi: 10.1038/260297a0. [DOI] [PubMed] [Google Scholar]

Articles from Proceedings of the National Academy of Sciences of the United States of America are provided here courtesy of National Academy of Sciences

RESOURCES