Skip to main content
NCBI home page
Proceedings of the National Academy of Sciences of the United States of America logoLink to Proceedings of the National Academy of Sciences of the United States of America
. 1981 Jan;78(1):279–282. doi: 10.1073/pnas.78.1.279

Formation of hybrid complexes between Ca2+ and the ionophores bromolasalocid (Br-X537A) and A23187

Michel Deleers *, Michel Gelbcke †, Willy J Malaisse *
PMCID: PMC319036  PMID: 16592950

Abstract

The ionophores A23187 and bromolasalocid (Br-X537A) acted synergistically in translocating Ca2+ from an aqueous into an organic immiscible phase or in mediating Ca2+ transport across the organic phase, the effects obtained in the simultaneous presence of both ionophores being greater than those expected from a summation of the individual effects of each ionophore. The nuclear magnetic resonance spectrum obtained when the complexation of Ca2+ occurred in the presence of both ionophores differed strikingly from the individual spectra obtained with the A23187-Ca and Br-X537A-Ca complexes. These findings indicate that the two ionophores are able to form hybrid complexes with Ca2+.

Keywords: nuclear magnetic resonance, synergism, ion translocation

Full text

PDF
279

Selected References

These references are in PubMed. This may not be the complete list of references from this article.

  1. Chaney M. O., Demarco P. V., Jones N. D., Occolowitz J. L. Letter: The structure of A23187, a divalent cation ionophore. J Am Chem Soc. 1974 Mar 20;96(6):1932–1933. doi: 10.1021/ja00813a047. [DOI] [PubMed] [Google Scholar]
  2. Couturier E., Deleers M., Malaisse W. J. Synergism between two distinct ionophores in translocating calcium from an aqueous to an organic environment. Pharmacol Res Commun. 1980 Jul;12(7):651–655. doi: 10.1016/s0031-6989(80)80102-0. [DOI] [PubMed] [Google Scholar]
  3. Couturier E., Malaisse W. J. Ionophore-mediated cation translocation in artificial systems. II. - X537A-mediated calcium and sodium translocation. Biochimie. 1980;62(2-3):177–180. doi: 10.1016/s0300-9084(80)80193-3. [DOI] [PubMed] [Google Scholar]
  4. Couturier E., Malaisse W. J. Synergistic effects of hypoglycaemic sulphonylureas and antibiotic ionophores upon calcium translocation. Br J Pharmacol. 1980;71(1):315–320. doi: 10.1111/j.1476-5381.1980.tb10941.x. [DOI] [PMC free article] [PubMed] [Google Scholar]
  5. Deber C. M., Pfieffer D. R. Ionophore A23187. Solution conformations of the calcium complex and free acid deduced from proton and carbon-13 nuclear magnetic resonance studies. Biochemistry. 1976 Jan 13;15(1):132–141. doi: 10.1021/bi00646a020. [DOI] [PubMed] [Google Scholar]
  6. Johnson S. M., Herrin J., Liu S. J., Paul I. C. The crystal and molecular structure of the barium salt of an antibiotic containing a high proportion of oxygen. J Am Chem Soc. 1970 Jul 15;92(14):4428–4435. doi: 10.1021/ja00717a047. [DOI] [PubMed] [Google Scholar]
  7. Malaisse V. J., Anjaneyulu K., Anjaneyulu R., Couturier E. Ionophore-mediated Ca2+ countertransport: role of Na+, Li+ or H+ gradient. Mol Cell Biochem. 1980 Apr 18;30(2):67–70. doi: 10.1007/BF00227919. [DOI] [PubMed] [Google Scholar]
  8. Malaisse W. J., Valverde I., Devis G., Somers G., Couturier E. Ionophore-mediated cation translocation in artificial systems. I. A23187-mediated calcium translocation. Biochimie. 1979;61(10):1185–1192. doi: 10.1016/s0300-9084(80)80232-x. [DOI] [PubMed] [Google Scholar]
  9. Patel D. J., Shen C. Structural and kinetic studies of lasalocid A (X537A) and its silver, sodium, and barium salts in nonpolar solvents. Proc Natl Acad Sci U S A. 1976 Jun;73(6):1786–1790. doi: 10.1073/pnas.73.6.1786. [DOI] [PMC free article] [PubMed] [Google Scholar]
  10. Pfeiffer D. R., Lardy H. A. Ionophore A23187: the effect of H+ concentration on complex formation with divalent and monovalent cations and the demonstration of K+ transport in mitochondria mediated by A23187. Biochemistry. 1976 Mar 9;15(5):935–943. doi: 10.1021/bi00650a001. [DOI] [PubMed] [Google Scholar]
  11. Reed P. W., Lardy H. A. A23187: a divalent cation ionophore. J Biol Chem. 1972 Nov 10;247(21):6970–6977. [PubMed] [Google Scholar]
  12. Shen C., Patel D. J. Free acid, anion, alkali, and alkaline earth complexes of lasalocid a (X537A) in methanol: structural and kinetic studies at the monomer level. Proc Natl Acad Sci U S A. 1976 Dec;73(12):4277–4281. doi: 10.1073/pnas.73.12.4277. [DOI] [PMC free article] [PubMed] [Google Scholar]

Articles from Proceedings of the National Academy of Sciences of the United States of America are provided here courtesy of National Academy of Sciences

RESOURCES