Skip to main content
NCBI home page
Proceedings of the National Academy of Sciences of the United States of America logoLink to Proceedings of the National Academy of Sciences of the United States of America
. 1981 Jan;78(1):358–362. doi: 10.1073/pnas.78.1.358

Serologically distinguishable alterations in the molecular specificity of cell cohesion during morphogenesis in Dictyostelium discoideum

David K Wilcox 1, Maurice Sussman 1
PMCID: PMC319052  PMID: 16592954

Abstract

Cells of the mutant strain JC-5 of the slime mold Dictyostelium discoideum exhibit at a specific, late developmental stage a temperature-sensitive morphogenetic defect associated with the loss of cell cohension. We show that at the restrictive temperature, the loss of cohesion and attendant dispersal of multicellular aggregates is associated with the disappearance or sequestration of a plasma membrane-bound moiety capable of reacting with and, hence, absorbing cohesion-blocking Fab. At the permissive temperature, the maintenance of cohesiveness past the critical stage or the recovery of lost cohesiveness is correlated with the presence or reappearance of the Fab-reactive moiety.

This moiety is absent or sterically incapable of reaction with Fab preparations at an earlier developmental stage in either mutant or wild-type cells—i.e., at a time when they have just entered into multicellular aggregates. Conversely, a serologically distinguishable membrane-bound moiety present in the early mutant or wild-type cells, whose reaction with homologous Fab also precludes their cohesion, is absent or serologically unreactive in either mutant or wild-type cells that are at comparable late developmental stages. We conclude that the cohesive moiety responsible for initiation of cell aggregates is supplanted by or transformed into a serologically distinct, cohesive complex responsible for the maintenance of the aggregate's integrity through the later stages of development.

Full text

PDF
358

Images in this article

360Image
on p.360
360Image
on p.360
360Image
on p.360
360Image
on p.360

Selected References

These references are in PubMed. This may not be the complete list of references from this article.

  1. Beug H., Gerisch G., Kempff S., Riedel V., Cremer G. Specific inhibition of cell contact formation in Dictyostelium by univalent antibodies. Exp Cell Res. 1970 Nov;63(1):147–158. doi: 10.1016/0014-4827(70)90343-5. [DOI] [PubMed] [Google Scholar]
  2. Beug H., Katz F. E., Gerisch G. Dynamics of antigenic membrane sites relating to cell aggregation in Dictyostelium discoideum. J Cell Biol. 1973 Mar;56(3):647–658. doi: 10.1083/jcb.56.3.647. [DOI] [PMC free article] [PubMed] [Google Scholar]
  3. Brackenbury R., Thiery J. P., Rutishauser U., Edelman G. M. Adhesion among neural cells of the chick embryo. I. An immunological assay for molecules involved in cell-cell binding. J Biol Chem. 1977 Oct 10;252(19):6835–6840. [PubMed] [Google Scholar]
  4. Feinberg A. P., Springer W. R., Barondes S. H. Segregation of pre-stalk and pre-spore cells of Dictyostelium discoideum: observations consistent with selective cell cohesion. Proc Natl Acad Sci U S A. 1979 Aug;76(8):3977–3981. doi: 10.1073/pnas.76.8.3977. [DOI] [PMC free article] [PubMed] [Google Scholar]
  5. Franke J., Sussman M. Accumulation of uridine diphosphoglucose pyrophosphorylase in Dictyostelium discoideum via preferential synthesis. J Mol Biol. 1973 Dec 5;81(2):173–185. doi: 10.1016/0022-2836(73)90187-3. [DOI] [PubMed] [Google Scholar]
  6. Frazier W. A., Rosen S. D., Reitherman R. W., Barondes S. H. Purification and comparison of two developmentally regulated lectins from Dictyostelium discoideum. Discoidin I and II. J Biol Chem. 1975 Oct 10;250(19):7714–7721. [PubMed] [Google Scholar]
  7. Green A. A., Newell P. C. The isolation and subfractionation of plasma membrane from the cellular slime mould Dictyostelium discoideum. Biochem J. 1974 May;140(2):313–322. doi: 10.1042/bj1400313. [DOI] [PMC free article] [PubMed] [Google Scholar]
  8. Kessin R. H. Mutations causing rapid development of Dictyostelium discoideum. Cell. 1977 Apr;10(4):703–708. doi: 10.1016/0092-8674(77)90104-0. [DOI] [PubMed] [Google Scholar]
  9. Moscona A. THE DEVELOPMENT IN VITRO OF CHIMERIC AGGREGATES OF DISSOCIATED EMBRYONIC CHICK AND MOUSE CELLS. Proc Natl Acad Sci U S A. 1957 Jan 15;43(1):184–194. doi: 10.1073/pnas.43.1.184. [DOI] [PMC free article] [PubMed] [Google Scholar]
  10. Müller K., Gerisch G. A specific glycoprotein as the target site of adhesion blocking Fab in aggregating Dictyostelium cells. Nature. 1978 Aug 3;274(5670):445–449. doi: 10.1038/274445a0. [DOI] [PubMed] [Google Scholar]
  11. Nestle M., Sussman M. The effect of cyclic AMP on morphogernesis and enzyme accumulation in Dictyostelium discoideum. Dev Biol. 1972 Aug;28(4):545–554. doi: 10.1016/0012-1606(72)90002-4. [DOI] [PubMed] [Google Scholar]
  12. Newell P. C., Franke J., Sussman M. Regulation of four functionally related enzymes during shifts in the developmental program of Dictyostelium discoideum. J Mol Biol. 1972 Feb 14;63(3):373–382. doi: 10.1016/0022-2836(72)90434-2. [DOI] [PubMed] [Google Scholar]
  13. Ray J., Shinnick T., Lerner R. A mutation altering the function of a carbohydrate binding protein blocks cell-cell cohesion in developing Dictyostelium discoideum. Nature. 1979 May 17;279(5710):215–221. doi: 10.1038/279215a0. [DOI] [PubMed] [Google Scholar]
  14. Reitherman R. W., Rosen S. D., Frasier W. A., Barondes S. H. Cell surface species-specific high affinity receptors for discoidin: developmental regulation in Dictyostelium discoideum. Proc Natl Acad Sci U S A. 1975 Sep;72(9):3541–3545. doi: 10.1073/pnas.72.9.3541. [DOI] [PMC free article] [PubMed] [Google Scholar]
  15. Rosen S. D., Kafka J. A., Simpson D. L., Barondes S. H. Developmentally regulated, carbohydrate-binding protein in Dictyostelium discoideum. Proc Natl Acad Sci U S A. 1973 Sep;70(9):2554–2557. doi: 10.1073/pnas.70.9.2554. [DOI] [PMC free article] [PubMed] [Google Scholar]
  16. SONNEBORN D. R., WHITE G. J., SUSSMAN M. A mutation affecting both rate and pattern of morphogenesis in Dictyostelium discoideum. Dev Biol. 1963 Mar;6:79–93. doi: 10.1016/0012-1606(63)90108-8. [DOI] [PubMed] [Google Scholar]
  17. Siu C. H., Lerner R. A., Ma G., Firtel R. A., Loomis W. F. Developmentally regulated proteins of the plasma membrane of Dictyostelium discoideum. The carbohydrate-binding protein. J Mol Biol. 1976 Jan 15;100(2):157–178. doi: 10.1016/s0022-2836(76)80146-5. [DOI] [PubMed] [Google Scholar]
  18. Steinemann C., Parish R. W. Evidence that a developmentally regulated glycoprotein is target of adhesion-blocking Fab in reaggregating Dictyostelium. Nature. 1980 Aug 7;286(5773):621–623. doi: 10.1038/286621a0. [DOI] [PubMed] [Google Scholar]
  19. Sussman M., Boschwitz C. Adhesive properties of cell ghosts derived from Dictyostelium discoideum. Dev Biol. 1975 Jun;44(2):362–368. doi: 10.1016/0012-1606(75)90406-6. [DOI] [PubMed] [Google Scholar]

Articles from Proceedings of the National Academy of Sciences of the United States of America are provided here courtesy of National Academy of Sciences

RESOURCES