VOLUME 284 (2009) PAGES 6079–6092
The experiments reported were carried out at 390 mm methyl-β-cyclodextrin (MβCD; 825 mg + 1 ml of water, which gives a volume of ∼1.6 ml), not as reported at 625 mm MβCD (825 mg/ml of solution). We (MiJin Son and E. L.) have investigated asymmetric vesicle preparation at the higher MβCD concentration and found that 625 mm MβCD could be used to produce asymmetric small unilamellar vesicles. However, the results were not as reproducible as with the lower MβCD concentration. We recommend the use of 390 mm MβCD to prepare asymmetric small unilamellar vesicles.