Skip to main content
NCBI home page
Proceedings of the National Academy of Sciences of the United States of America logoLink to Proceedings of the National Academy of Sciences of the United States of America
. 1981 Jan;78(1):592–596. doi: 10.1073/pnas.78.1.592

Characterization of angiotensin receptors on bovine adrenal fasciculata cells.

M B Vallotton, A M Capponi, C Grillet, A L Knupfer, R Hepp, M C Khosla, F M Bumpus
PMCID: PMC319100  PMID: 6264451

Abstract

We have further characterized angiotensin receptors on bovine adrenal fasciculata cells whose presence was previously demonstrated by the intrinsic agonistic activity of angiotensin II (AII), dex-Asp1-AII, angiotensin I (AI), and des-ASp1-AI on steroidogenesis. The specific binding of AII and des-Asp1-AII labeled with 125I to dispersed bovine fasciculata cells was studied. For both peptides, a single class of binding sites accounted for the data with a mean (+/- SEM) Ka value of 0.23 +/- 0.123 X 10(8) liters/mol for AII and 0.68 X 10(8) liters/mol for des-Asp1-AII. The concentration at which unlabeled AII and des-Asp1-AII displaced 50% of the tracers (Kd) was similar to that at which they induced half-maximal stimulation of steroidogenesis (Kact). For AI and des-Asp1-AI, Kd greater than Kact. Analogs of AII or des-Asp1-AII with antagonistic properties upon steroidogenesis competed also with binding of the tracers. Corticotropin (ACTH) did not inhibit binding. Although ACTH stimulated the formation of cyclic AMP, none of the angiotensins with intrinsic activity did so. Calcium, but not potassium, appeared to potentiate the steroidogenic activity of AII. These data suggest that there is a single class of receptors for angiotensins and analogs in zona fasciculata. These receptors show characteristics that differentiate them from ACTH receptors in zona fasciculata or angiotensin receptors in zona glomerulosa cells.

Full text

PDF
592

Selected References

These references are in PubMed. This may not be the complete list of references from this article.

  1. AMES R. P., BORKOWSKI A. J., SICINSKI A. M., LARAGH J. H. PROLONGED INFUSIONS OF ANGIOTENSIN II AND NOREPINEPHRINE AND BLOOD PRESSURE, ELECTROLYTE BALANCE, AND ALDOSTERONE AND CORTISOL SECRETION IN NORMAL MAN AND IN CIRRHOSIS WITH ASCITES. J Clin Invest. 1965 Jul;44:1171–1186. doi: 10.1172/JCI105224. [DOI] [PMC free article] [PubMed] [Google Scholar]
  2. Aguilera G., Capponi A., Baukal A., Fujita K., Hauger R., Catt K. J. Metabolism and biological activities of angiotensin II and des-Asp1-angiotensin II in isolated adrenal glomerulosa cells. Endocrinology. 1979 May;104(5):1279–1285. doi: 10.1210/endo-104-5-1279. [DOI] [PubMed] [Google Scholar]
  3. Aguilera G., Catt K. J. Loci of action of regulators of aldosterone biosynthesis in isolated glomerulosa cells. Endocrinology. 1979 Apr;104(4):1046–1052. doi: 10.1210/endo-104-4-1046. [DOI] [PubMed] [Google Scholar]
  4. Albano J. D., Brown B. L., Ekins R. P., Tait S. A., Tait J. F. The effects of potassium, 5-hydrocytryptamine, adrenocorticotrophin and angiotensin II on the concentration of adenosine 3':5'-cyclic monophosphate in suspensions of dispersed rat adrenal zona glomerulosa and zona fasciculata cells. Biochem J. 1974 Aug;142(2):391–400. doi: 10.1042/bj1420391. [DOI] [PMC free article] [PubMed] [Google Scholar]
  5. Bing R. F., Schulster D. Adenosine 3':5'-cyclic monophosphate production and steroidogenesis by isolated rat adrenal glomerulosa cells. Effects of angiotensin II and [Sar 1,Ala 8]angiotensin II. Biochem J. 1978 Oct 15;176(1):39–45. doi: 10.1042/bj1760039. [DOI] [PMC free article] [PubMed] [Google Scholar]
  6. Bing R. F., Schulster D. Steroidogenesis in isolated rat adrenal glomerulosa cells: response to physiological concentrations of angiotensin II and effects of potassium, serotonin and [Sar1,Ala8]-angiotensin II. J Endocrinol. 1977 Aug;74(2):261–272. doi: 10.1677/joe.0.0740261. [DOI] [PubMed] [Google Scholar]
  7. Bravo E. L., Khosla M. C., Bumpus F. M. Vascular and adrenocortical responses to a specific antagonist of angiotensin II. Am J Physiol. 1975 Jan;228(1):110–114. doi: 10.1152/ajplegacy.1975.228.1.110. [DOI] [PubMed] [Google Scholar]
  8. CARPENTER C. C., DAVIS J. O., AYERS C. R. Relation of renin, angiotensin II, and experimental renal hypertension to aldosterone secretion. J Clin Invest. 1961 Nov;40:2026–2042. doi: 10.1172/JCI104429. [DOI] [PMC free article] [PubMed] [Google Scholar]
  9. Capponi A. M., Catt K. J. Angiotensin II receptors in adrenal cortex and uterus. Binding and activation properties of angiotensin analogues. J Biol Chem. 1979 Jun 25;254(12):5120–5127. [PubMed] [Google Scholar]
  10. Devynck M. A., Pernollet M. G., Matthews P. G., Khosla M. C., Bumpus F. M., Meyer P. Specific receptors for des-Asp1-angiotensin II (("angiotensin III") in rat adrenals. Proc Natl Acad Sci U S A. 1977 Sep;74(9):4029–4032. doi: 10.1073/pnas.74.9.4029. [DOI] [PMC free article] [PubMed] [Google Scholar]
  11. Douglas J. G., Michailov M., Khosla M. C., Bumpus F. M. Comparative studies of receptor binding and steroidogenic properties of angiotensins in the rat adrenal glomerulosa. Endocrinology. 1979 Jan;104(1):71–75. doi: 10.1210/endo-104-1-71. [DOI] [PubMed] [Google Scholar]
  12. Douglas J., Bartley P., Kondo T., Catt K. Formation of DES-ASP1-angiotensin II is not an obligatory step in the steroidogenic action of angiotensin II in the canine adrenal. Endocrinology. 1978 Jun;102(6):1921–1924. doi: 10.1210/endo-102-6-1921. [DOI] [PubMed] [Google Scholar]
  13. Espiner E. A., Livesey J. H., Ross J., Donald R. A. Dynamics of cyclic adenosine 3',5'-monophosphate release during adrenocortical stimulation in vivo. Endocrinology. 1974 Sep;95(3):838–846. doi: 10.1210/endo-95-3-838. [DOI] [PubMed] [Google Scholar]
  14. Fakunding J. L., Chow R., Catt K. J. The role of calcium in the stimulation of aldosterone production by adrenocorticotropin, angiotensin II, and potassium in isolated glomerulosa cells. Endocrinology. 1979 Aug;105(2):327–333. doi: 10.1210/endo-105-2-327. [DOI] [PubMed] [Google Scholar]
  15. Fujita K., Aguilera G., Catt K. J. The role of cyclic AMP in aldosterone production by isolated zona glomerulosa cells. J Biol Chem. 1979 Sep 10;254(17):8567–8574. [PubMed] [Google Scholar]
  16. Gilman A. G. A protein binding assay for adenosine 3':5'-cyclic monophosphate. Proc Natl Acad Sci U S A. 1970 Sep;67(1):305–312. doi: 10.1073/pnas.67.1.305. [DOI] [PMC free article] [PubMed] [Google Scholar]
  17. Glossmann H., Baukal A. J., Catt K. J. Properties of angiotensin II receptors in the bovine and rat adrenal cortex. J Biol Chem. 1974 Feb 10;249(3):825–834. [PubMed] [Google Scholar]
  18. HUNTER W. M., GREENWOOD F. C. Preparation of iodine-131 labelled human growth hormone of high specific activity. Nature. 1962 May 5;194:495–496. doi: 10.1038/194495a0. [DOI] [PubMed] [Google Scholar]
  19. Haning R., Tait S. A., Tait J. F. In vitro effects of ACTH, angiotensins, serotonin and potassium on steroid output and conversion of corticosterone to aldosterone by isolated adrenal cells. Endocrinology. 1970 Dec;87(6):1147–1167. doi: 10.1210/endo-87-6-1147. [DOI] [PubMed] [Google Scholar]
  20. Hepp R., Grillet C., Peytremann A., Vallotton M. B. Stimulating effects of angiotensin I, angiotensin II and des-Asp1-angiotensin II on steroid production in vitro and its inhibition by Sar1-Ala8-angiotensin II. Prog Biochem Pharmacol. 1976;12:41–48. [PubMed] [Google Scholar]
  21. Hepp R., Grillet C., Peytremann A., Vallotton M. B. Stimulation of corticosteroid biosynthesis by angiotensin I [des-asp1]angiotensin I, angiotensin II and [des-asp1]-angiotensin II in bovine adrenal fasciculata cells. Endocrinology. 1977 Sep;101(3):717–725. doi: 10.1210/endo-101-3-717. [DOI] [PubMed] [Google Scholar]
  22. KAPLAN N. M., BARTER F. C. The effect of ACTH, renin, angiotensin II, and various precursors on biosynthesis of aldosterone by adrenal slices. J Clin Invest. 1962 Apr;41:715–724. doi: 10.1172/JCI104530. [DOI] [PMC free article] [PubMed] [Google Scholar]
  23. Kaplan N. M. The biosynthesis of adrenal steroids: effects of angiotensin II, adrenocorticotropin, and potassium. J Clin Invest. 1965 Dec;44(12):2029–2039. doi: 10.1172/JCI105310. [DOI] [PMC free article] [PubMed] [Google Scholar]
  24. Kloppenborg P. W., Island D. P., Liddle G. W., Michelakis A. M., Nicholson W. E. A method of preparing adrenal cell suspensions and its applicability to the in vitro study of adrenal metabolism. Endocrinology. 1968 May;82(5):1053–1058. doi: 10.1210/endo-82-5-1053. [DOI] [PubMed] [Google Scholar]
  25. Leclercq R., Copinschi G., Franckson J. R. Le dosage par compétition du cortisol plasmatique. Modification de la méthode de Murphy. Rev Fr Etud Clin Biol. 1969 Oct;14(8):815–819. [PubMed] [Google Scholar]
  26. Lobo M. V., Marusic E. T., Aguilera G. Further studies on the relationship between potassium and sodium levels and adrenocortical activity. Endocrinology. 1978 Apr;102(4):1061–1068. doi: 10.1210/endo-102-4-1061. [DOI] [PubMed] [Google Scholar]
  27. Peytremann A., Nicholson W. E., Brown R. D., Liddle G. W., Hardman J. G. Comparative effects of angiotensin and ACTH on cyclic AMP and steroidogenesis in isolated bovine adrenal cells. J Clin Invest. 1973 Apr;52(4):835–842. doi: 10.1172/JCI107247. [DOI] [PMC free article] [PubMed] [Google Scholar]
  28. SLATER J. D., BARBOUR B. H., HENDERSON H. H., CASPER A. G., BARTTER F. C. INFLUENCE OF THE PITUITARY AND THE RENIN-ANGIOTENSIN SYSTEM ON THE SECRETION OF ALDOSTERONE, CORTISOL, AND CORTICOSTERONE. J Clin Invest. 1963 Sep;42:1504–1520. doi: 10.1172/JCI104835. [DOI] [PMC free article] [PubMed] [Google Scholar]
  29. Saltman S., Fredlund P., Catt K. J. Actions of angiotensin II antagonists upon aldosterone production by isolated adrenal glomerulosa cells. Endocrinology. 1976 Apr;98(4):894–903. doi: 10.1210/endo-98-4-894. [DOI] [PubMed] [Google Scholar]
  30. Saruta T., Cook R., Kaplan N. M. Adrenocortical steroidogenesis: studies on the mechanism of action of angiotensin and electrolytes. J Clin Invest. 1972 Sep;51(9):2239–2245. doi: 10.1172/JCI107032. [DOI] [PMC free article] [PubMed] [Google Scholar]
  31. Sayers G., Swallow R. L., Giordano N. D. An improved technique for the preparation of isolated rat adrenal cells: a sensitive, accurate and specfic method for the assay of ACTH. Endocrinology. 1971 Apr;88(4):1063–1068. doi: 10.1210/endo-88-4-1063. [DOI] [PubMed] [Google Scholar]
  32. Shima S., Kawashima Y., Hirai M. Studies on cyclic nucleotides in the adrenal gland. VIII. Effects of angiotensin on adenosine 3',5'-monophosphate and steroidogenesis in the adrenal cortex. Endocrinology. 1978 Oct;103(4):1361–1367. doi: 10.1210/endo-103-4-1361. [DOI] [PubMed] [Google Scholar]

Articles from Proceedings of the National Academy of Sciences of the United States of America are provided here courtesy of National Academy of Sciences

RESOURCES