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. 1981 Mar;78(3):1466–1470. doi: 10.1073/pnas.78.3.1466

Characterization of a cDNA clone coding for the beta chain of bovine fibrinogen.

D W Chung, M W Rixon, R T MacGillivray, E W Davie
PMCID: PMC319151  PMID: 6262803

Abstract

Recombinant plasmids containing bovine cDNA have been screened with a radiolabeled cDNA enriched for bovine fibrinogen. A number of plasmids containing cDNAs for fibrinogen were identified by this assay. One plasmid, designated pBI beta 1, was found to contain a cDNA insert of 1372 base pairs. The sequence of the cDNA insert for this plasmid was then determined. It was shown to code for 424 amino acids of the beta chain of fibrinogen, starting with residue 44. This and other data made it possible to construct the complete amino acid sequence of the beta chain of the protein. Comparison of the amino acid sequence of the beta chain of bovine fibrinogen with the corresponding chain of the human molecule indicated that the two chains are greater than 80% homologous.

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Selected References

These references are in PubMed. This may not be the complete list of references from this article.

  1. Blombäck B., Blombäck M. The molecular structure of fibrinogen. Ann N Y Acad Sci. 1972 Dec 8;202:77–97. doi: 10.1111/j.1749-6632.1972.tb16323.x. [DOI] [PubMed] [Google Scholar]
  2. Blombäck B., Hessel B., Hogg D. Disulfide bridges in nh2 -terminal part of human fibrinogen. Thromb Res. 1976 May;8(5):639–658. doi: 10.1016/0049-3848(76)90245-0. [DOI] [PubMed] [Google Scholar]
  3. Blombäck B., Hogg D. H., Gårdlund B., Hessel B., Kudryk B. Fibrinogen and fibrin formation. Thromb Res. 1976 May;8(2 Suppl):329–346. doi: 10.1016/0049-3848(76)90075-x. [DOI] [PubMed] [Google Scholar]
  4. Blombäck B. Nomenclature of fibrinogen and fibrin chains and other derivatives of fibrinogen and fibrin. Thromb Diath Haemorrh Suppl. 1968;35:161–168. [PubMed] [Google Scholar]
  5. CLEGG J. B., BAILEY K. The separation and isolation of the peptide chains of fibrin. Biochim Biophys Acta. 1962 Oct 8;63:525–527. doi: 10.1016/0006-3002(62)90122-1. [DOI] [PubMed] [Google Scholar]
  6. Chung D. W., MacGillivray R. T., Davie E. W. The biosynthesis of bovine fibrinogen, prothrombin, and albumin in a cell-free system. Ann N Y Acad Sci. 1980;343:210–217. doi: 10.1111/j.1749-6632.1980.tb47253.x. [DOI] [PubMed] [Google Scholar]
  7. Donovan J. W., Mihalyi E. Conformation of fibrinogen: calorimetric evidence for a three-nodular structure. Proc Natl Acad Sci U S A. 1974 Oct;71(10):4125–4128. doi: 10.1073/pnas.71.10.4125. [DOI] [PMC free article] [PubMed] [Google Scholar]
  8. Doolittle R. F., Goldbaum D. M., Doolittle L. R. Designation of sequences involved in the "coiled-coil" interdomainal connections in fibrinogen: constructions of an atomic scale model. J Mol Biol. 1978 Apr 5;120(2):311–325. doi: 10.1016/0022-2836(78)90070-0. [DOI] [PubMed] [Google Scholar]
  9. Doolittle R. F. Structural aspects of the fibrinogen to fibrin conversion. Adv Protein Chem. 1973;27:1–109. doi: 10.1016/s0065-3233(08)60446-5. [DOI] [PubMed] [Google Scholar]
  10. Doolittle R. F., Watt K. W., Cottrell B. A., Strong D. D., Riley M. The amino acid sequence of the alpha-chain of human fibrinogen. Nature. 1979 Aug 9;280(5722):464–468. doi: 10.1038/280464a0. [DOI] [PubMed] [Google Scholar]
  11. FOLK J. E., GLADNER J. A., LEVIN Y. Thrombin-induced formation of co-fibrin. III. Acid degradation studies and summary of sequential evidence on peptide A. J Biol Chem. 1959 Sep;234:2317–2320. [PubMed] [Google Scholar]
  12. FORMAN W. B., BARNHART M. I. CELLULAR SITE FOR FIBRINOGEN SYNTHESIS. JAMA. 1964 Jan 11;187:128–132. [PubMed] [Google Scholar]
  13. Fowler W. E., Erickson H. P. Trinodular structure of fibrinogen. Confirmation by both shadowing and negative stain electron microscopy. J Mol Biol. 1979 Oct 25;134(2):241–249. doi: 10.1016/0022-2836(79)90034-2. [DOI] [PubMed] [Google Scholar]
  14. Gough N. M., Adams J. M. Immunoprecipitation of specific polysomes using Staphylococcus aureus: purification of the immunoglobulin k chain messenger RNA from the mouse myeloma MPC11. Biochemistry. 1978 Dec 12;17(25):5560–5566. doi: 10.1021/bi00618a036. [DOI] [PubMed] [Google Scholar]
  15. HALL C. E., SLAYTER H. S. The fibrinogen molecule: its size, shape, and mode of polymerization. J Biophys Biochem Cytol. 1959 Jan 25;5(1):11–16. doi: 10.1083/jcb.5.1.11. [DOI] [PMC free article] [PubMed] [Google Scholar]
  16. Henschen A., Lottspeich F. Amino acid sequence of human fibrin. Preliminary note on the completion of the beta-chain sequence. Hoppe Seylers Z Physiol Chem. 1977 Dec;358(12):1643–1646. [PubMed] [Google Scholar]
  17. Lottspeich F., Henschen A. Amino acid sequence of human fibrin. Preliminary note on the completion of the gamma-chain sequence. Hoppe Seylers Z Physiol Chem. 1977 Jul;358(7):935–938. [PubMed] [Google Scholar]
  18. MacGillivray R. T., Degen S. J., Chandra T., Woo S. L., Davie E. W. Cloning and analysis of a cDNA coding for bovine prothrombin. Proc Natl Acad Sci U S A. 1980 Sep;77(9):5153–5157. doi: 10.1073/pnas.77.9.5153. [DOI] [PMC free article] [PubMed] [Google Scholar]
  19. Maniatis T., Jeffrey A., van deSande H. Chain length determination of small double- and single-stranded DNA molecules by polyacrylamide gel electrophoresis. Biochemistry. 1975 Aug 26;14(17):3787–3794. doi: 10.1021/bi00688a010. [DOI] [PubMed] [Google Scholar]
  20. Martinelli R. A., Inglis A. S., Rubira M. R., Hageman T. C., Hurrell J. G., Leach S. J., Scheraga H. A. Amino acid sequences of portions of the alpha and beta chains of bovine fibrinogen. Arch Biochem Biophys. 1979 Jan;192(1):27–32. doi: 10.1016/0003-9861(79)90068-7. [DOI] [PubMed] [Google Scholar]
  21. Maxam A. M., Gilbert W. Sequencing end-labeled DNA with base-specific chemical cleavages. Methods Enzymol. 1980;65(1):499–560. doi: 10.1016/s0076-6879(80)65059-9. [DOI] [PubMed] [Google Scholar]
  22. McGillivray R. T., Chung D. W., Davie E. W. Biosynthesis of bovine plasma proteins in a cell-free system. Amino-terminal sequence of preproalbumin. Eur J Biochem. 1979 Aug 1;98(2):477–485. doi: 10.1111/j.1432-1033.1979.tb13209.x. [DOI] [PubMed] [Google Scholar]
  23. McKee P. A., Rogers L. A., Marler E., Hill R. L. The subunit polypeptides of human fibrinogen. Arch Biochem Biophys. 1966 Sep 26;116(1):271–279. doi: 10.1016/0003-9861(66)90033-6. [DOI] [PubMed] [Google Scholar]
  24. Smith A. J. DNA sequence analysis by primed synthesis. Methods Enzymol. 1980;65(1):560–580. doi: 10.1016/s0076-6879(80)65060-5. [DOI] [PubMed] [Google Scholar]
  25. Smith H. O., Birnstiel M. L. A simple method for DNA restriction site mapping. Nucleic Acids Res. 1976 Sep;3(9):2387–2398. doi: 10.1093/nar/3.9.2387. [DOI] [PMC free article] [PubMed] [Google Scholar]
  26. Smith M., Leung D. W., Gillam S., Astell C. R., Montgomery D. L., Hall B. D. Sequence of the gene for iso-1-cytochrome c in Saccharomyces cerevisiae. Cell. 1979 Apr;16(4):753–761. doi: 10.1016/0092-8674(79)90091-6. [DOI] [PubMed] [Google Scholar]
  27. Staden R. Sequence data handling by computer. Nucleic Acids Res. 1977 Nov;4(11):4037–4051. doi: 10.1093/nar/4.11.4037. [DOI] [PMC free article] [PubMed] [Google Scholar]
  28. Sutcliffe J. G. Nucleotide sequence of the ampicillin resistance gene of Escherichia coli plasmid pBR322. Proc Natl Acad Sci U S A. 1978 Aug;75(8):3737–3741. doi: 10.1073/pnas.75.8.3737. [DOI] [PMC free article] [PubMed] [Google Scholar]
  29. Szalay A. A., Grohmann K., Sinsheimer R. L. Separation of the complementary strands of DNA fragments on polyacrylamide gels. Nucleic Acids Res. 1977;4(5):1569–1578. doi: 10.1093/nar/4.5.1569. [DOI] [PMC free article] [PubMed] [Google Scholar]
  30. Töpfer-Peterson E., Lottspeich F., Henschen A. Carbohydrate linkage site in the beta-chain of human fibrin. Hoppe Seylers Z Physiol Chem. 1976 Nov;357(11):1509–1513. doi: 10.1515/bchm2.1976.357.2.1509. [DOI] [PubMed] [Google Scholar]
  31. Watt K. W., Takagi T., Doolittle R. F. Amino acid sequence of the beta chain of human fibrinogen. Biochemistry. 1979 Jan 9;18(1):68–76. doi: 10.1021/bi00568a011. [DOI] [PubMed] [Google Scholar]

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