Skip to main content
. Author manuscript; available in PMC: 2012 Mar 15.
Published in final edited form as: Biochemistry. 2011 Feb 9;50(10):1700–1713. doi: 10.1021/bi101977w

Figure 6.

Figure 6

P4H1 and Gnt1 modify animal Skp1s. Skp1 isoforms from C. elegans (Skr1 and Skr2) and H. sapiens (OCP-2, or isoform B), and mutant OCP-2(E147P), were expressed as N-terminally FLAG-tagged proteins in E. coli and purified by means of their FLAG-tags. (A) SDS-PAGE analysis followed by staining with Coomassie blue for total protein. (B) Analysis of P4H1 acceptor activity of 0.31 µM FLAG-Skp1’s using the coupled, 1-step 3H-assay. Data are representative of 2 independent trials at different concentrations.