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. 2011 May 24;152(7):2870–2882. doi: 10.1210/en.2011-0219

Fig. 5.

Fig. 5.

Expression of steroidogenic pathway genes and steroids in SF-1-ES cells differentiated in the absence of LIF. In presence of SF-1, ES cells differentiate in LIF(−) media to form a proliferating cell population capable of effective steroidogenesis upon cAMP induction. A, Schematic illustration of the protocol for differentiation of SF-1-ES cells into a steroidogenic cell population. B, Steroidogenesis in the differentiated SF-1-ES cells after cAMP treatment as assessed by progesterone, testosterone, and estradiol production. C, Real-time RT-PCR analysis characterizing steroidogenic pathway gene induction in differentiated SF-1-ES cells after cAMP treatment. Relative mRNA expression levels were calibrated to 18S RNA. Values are means ± sd (n = 3). *, P ≤ 0.05. ND, Not detectable.