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Proceedings of the National Academy of Sciences of the United States of America logoLink to Proceedings of the National Academy of Sciences of the United States of America
. 1981 Mar;78(3):1901–1905. doi: 10.1073/pnas.78.3.1901

Enzyme-linked sandwich immunoassay for insulin using laser fluorimetric detection.

S D Lidofsky, W D Hinsberg 3rd, R N Zare
PMCID: PMC319243  PMID: 7015349

Abstract

Human serum samples are assayed for insulin by an enzyme-linked sandwich immunoassay. Horseradish peroxidase is used as an enzyme label for antibody, and enzyme activity is measured by means of the fluorogenic substrate, p-hydroxyphenylacetic acid. The product is detected by excitation of fluorescence with the 325-nm line of a continuous-wave helium/cadmium ion laser on line with reverse-phase high-pressure liquid chromatography. The incubation period is 90 min and the limit of detection of insulin is 30 pM, corresponding to 5 microunits/ml. This method correlates highly with radioimmunoassay, with coefficient of correlation r = 0.95.

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Selected References

These references are in PubMed. This may not be the complete list of references from this article.

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