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. 1981 Apr;78(4):2100–2104. doi: 10.1073/pnas.78.4.2100

recA protein promotes homologous-pairing and strand-exchange reactions between duplex DNA molecules.

S C West, E Cassuto, P Howard-Flanders
PMCID: PMC319291  PMID: 6941272

Abstract

Purified recA protein, product of the recA+ gene, promotes homologous pairing between intact covalent circular duplex DNA and circular single-stranded DNA carrying a short hybridized fragment [West, S. C., Cassuto, E. & Howard-Flanders, P. (1981) Nature (London) 290, 29-33.]. In this paper we investigate the interaction of duplex fragments with circular single-stranded DNA carrying the hybridized fragment and find that recA protein promotes an efficient strand-exchange reaction between interacting DNA molecules. The exchange is dependent upon linear duplex DNA fragments that are homologous to, but extend beyond, the short fragment present on the hybridized DNA substrate. The reactions require stoichiometric amounts of recA protein and the presence of ATP.

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Selected References

These references are in PubMed. This may not be the complete list of references from this article.

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