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. 1981 Apr;78(4):2110–2114. doi: 10.1073/pnas.78.4.2110

Amplification of the metallothionein-I gene in cadmium-resistant mouse cells.

L R Beach, R D Palmiter
PMCID: PMC319293  PMID: 6941274

Abstract

Friend leukemia cells resistant to cadmium toxicity were selected. More than 70% of total cysteine incorporation in these cells was into the metal-binding protein, metallothionein. We used cDNA and genomic DNA clones containing the metallothionein-I gene to measure the concentration of its mRNA, the rate of gene transcription, and the number of genes. On a per cell basis, optimally induced, cadmium-resistant cells have a 14-fold more metallothionein-I mRNA, a 6-fold higher rate of metallothionein-I gene transcription, and 6-fold more metallothionein-I genes than do nonresistant cells. Metaphase spreads revealed that the resistant cells are nearly tetraploid and contain, on the average, three very small chromosomes that are absent from non-resistant Friend cells.

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Selected References

These references are in PubMed. This may not be the complete list of references from this article.

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