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. 1981 Apr;78(4):2130–2134. doi: 10.1073/pnas.78.4.2130

Distribution of protein I in mammalian brain as determined by a detergent-based radioimmunoassay.

S E Goelz, E J Nestler, B Chehrazi, P Greengard
PMCID: PMC319297  PMID: 6787599

Abstract

A radioimmunoassay has been developed for measuring protein I, a basic, neuron-specific protein associated with nerve terminals. The procedure utilizes the detergents NaDodSO4 and Nonidet P-40 to prevent nonspecific adsorption of this highly charged protein to various surfaces. By use of this procedure, it has been possible to show that protein I comprises approximately 0.4% of the total protein in cerebral cortex of several mammalian species. In addition, the amount of protein I was determined in about 40 regions of cat brain. The results suggest that measurement of protein I may provide a quantitative method for estimating the density of nerve terminals in various regions of the mammalian nervous system.

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Selected References

These references are in PubMed. This may not be the complete list of references from this article.

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