Skip to main content
NCBI home page
Proceedings of the National Academy of Sciences of the United States of America logoLink to Proceedings of the National Academy of Sciences of the United States of America
. 1981 Apr;78(4):2145–2149. doi: 10.1073/pnas.78.4.2145

A topographic gradient of molecules in retina can be used to identify neuron position.

G D Trisler, M D Schneider, M Nirenberg
PMCID: PMC319300  PMID: 6166002

Abstract

A monoclonal antibody was obtained that binds to cell membrane molecules distributed in a topographic gradient in avian retina. Thirty-five-fold more antigen was detected in dorsoposterior retina that in ventroanterior retina. Most of the antigen was associated with the synaptic layers of the retina. Less antigen was detected in cerebrum, thalamus, cerebellum, and optic tectum, but little or none was found in non-neural tissues tested. The antigen was found on most or all cell types in retina, and the concentration of antigen found is a function of the square of the circumferential distance from the ventroanterior pole of the gradient toward the dorsoposterior pole. Thus, the antigen can be used as a marker of cell position along the ventroanterior-dorsoposterior axis of the retina.

Full text

PDF
2145

Images in this article

2147Image
on p.2147
2148Image
on p.2148

Selected References

These references are in PubMed. This may not be the complete list of references from this article.

  1. Barbera A. J., Marchase R. B., Roth S. Adhesive recognition and retinotectal specificity. Proc Natl Acad Sci U S A. 1973 Sep;70(9):2482–2486. doi: 10.1073/pnas.70.9.2482. [DOI] [PMC free article] [PubMed] [Google Scholar]
  2. Barnstable C. J. Monoclonal antibodies which recognize different cell types in the rat retina. Nature. 1980 Jul 17;286(5770):231–235. doi: 10.1038/286231a0. [DOI] [PubMed] [Google Scholar]
  3. Berzofsky J. A., Hicks G., Fedorko J., Minna J. Properties of monoclonal antibodies specific for determinants of a protein antigen, myoglobin. J Biol Chem. 1980 Dec 10;255(23):11188–11191. [PubMed] [Google Scholar]
  4. Bolton A. E., Hunter W. M. The labelling of proteins to high specific radioactivities by conjugation to a 125I-containing acylating agent. Biochem J. 1973 Jul;133(3):529–539. doi: 10.1042/bj1330529. [DOI] [PMC free article] [PubMed] [Google Scholar]
  5. Cafferata R., Panosian J., Bordley G. Developmental and biochemical studies of adhesive specificity among embryonic retinal cells. Dev Biol. 1979 Mar;69(1):108–117. doi: 10.1016/0012-1606(79)90278-1. [DOI] [PubMed] [Google Scholar]
  6. Eisenbarth G. S., Walsh F. S., Nirenberg M. Monoclonal antibody to a plasma membrane antigen of neurons. Proc Natl Acad Sci U S A. 1979 Oct;76(10):4913–4917. doi: 10.1073/pnas.76.10.4913. [DOI] [PMC free article] [PubMed] [Google Scholar]
  7. Galfre G., Howe S. C., Milstein C., Butcher G. W., Howard J. C. Antibodies to major histocompatibility antigens produced by hybrid cell lines. Nature. 1977 Apr 7;266(5602):550–552. doi: 10.1038/266550a0. [DOI] [PubMed] [Google Scholar]
  8. Galifret Y. Les diverses aires fonctionnelles de la rétine du pigeon. Z Zellforsch Mikrosk Anat. 1968;86(4):535–545. [PubMed] [Google Scholar]
  9. Goldschneider I., Moscona A. A. Tissue-specific cell-surface antigens in embryonic cells. J Cell Biol. 1972 May;53(2):435–449. doi: 10.1083/jcb.53.2.435. [DOI] [PMC free article] [PubMed] [Google Scholar]
  10. Gottlieb D. I., Rock K., Glaser L. A gradient of adhesive specificity in developing avian retina. Proc Natl Acad Sci U S A. 1976 Feb;73(2):410–414. doi: 10.1073/pnas.73.2.410. [DOI] [PMC free article] [PubMed] [Google Scholar]
  11. HAMDI F. A., WHITTERIDGE D. The representation of the retina on the optic tectum of the pigeon. Q J Exp Physiol Cogn Med Sci. 1954;39(2):111–119. doi: 10.1113/expphysiol.1954.sp001053. [DOI] [PubMed] [Google Scholar]
  12. Hausman R. E., Moscona A. A. Immunologic detection of retina cognin on the surface of embryonic cells. Exp Cell Res. 1979 Mar 15;119(2):191–204. doi: 10.1016/0014-4827(79)90348-3. [DOI] [PubMed] [Google Scholar]
  13. Hirose G., Jacobson M. Clonal organization of the central nervous system of the frog. I. Clones stemming from individual blastomeres of the 16-cell and earlier stages. Dev Biol. 1979 Aug;71(2):191–202. doi: 10.1016/0012-1606(79)90163-5. [DOI] [PubMed] [Google Scholar]
  14. Köhler G., Milstein C. Continuous cultures of fused cells secreting antibody of predefined specificity. Nature. 1975 Aug 7;256(5517):495–497. doi: 10.1038/256495a0. [DOI] [PubMed] [Google Scholar]
  15. LOWRY O. H., ROSEBROUGH N. J., FARR A. L., RANDALL R. J. Protein measurement with the Folin phenol reagent. J Biol Chem. 1951 Nov;193(1):265–275. [PubMed] [Google Scholar]
  16. Marchase R. B. Biochemical investigations of retinotectal adhesive specificity. J Cell Biol. 1977 Oct;75(1):237–257. doi: 10.1083/jcb.75.1.237. [DOI] [PMC free article] [PubMed] [Google Scholar]
  17. McGill J. I., Powell T. P., Cowan W. M. The retinal representation upon the optic tectum and isthmo-optic nucleus in the pigeon. J Anat. 1966 Jan;100(Pt 1):5–33. [PMC free article] [PubMed] [Google Scholar]
  18. Rutz R., Lilien J. Functional characterization of an adhesive component from the embryonic chick neural retina. J Cell Sci. 1979 Apr;36:323–342. doi: 10.1242/jcs.36.1.323. [DOI] [PubMed] [Google Scholar]
  19. SPERRY R. W. CHEMOAFFINITY IN THE ORDERLY GROWTH OF NERVE FIBER PATTERNS AND CONNECTIONS. Proc Natl Acad Sci U S A. 1963 Oct;50:703–710. doi: 10.1073/pnas.50.4.703. [DOI] [PMC free article] [PubMed] [Google Scholar]
  20. Sanyal S., Zeilmaker G. H. Cell lineage in retinal development of mice studied in experimental chimaeras. Nature. 1977 Feb 24;265(5596):731–733. doi: 10.1038/265731a0. [DOI] [PubMed] [Google Scholar]
  21. Schneider M. D., Eisenbarth G. S. Transfer plate radioassay using cell monolayers to detect anti-cell surface antibodies synthesized by lymphocyte hybridomas. J Immunol Methods. 1979;29(4):331–342. doi: 10.1016/0022-1759(79)90004-8. [DOI] [PubMed] [Google Scholar]
  22. Silver J., Sidman R. L. A mechanism for the guidance and topographic patterning of retinal ganglion cell axons. J Comp Neurol. 1980 Jan 1;189(1):101–111. doi: 10.1002/cne.901890106. [DOI] [PubMed] [Google Scholar]
  23. Thiery J. P., Brackenbury R., Rutishauser U., Edelman G. M. Adhesion among neural cells of the chick embryo. II. Purification and characterization of a cell adhesion molecule from neural retina. J Biol Chem. 1977 Oct 10;252(19):6841–6845. [PubMed] [Google Scholar]
  24. Trisler G. D., Donlon M. A., Shain W. G., Coon H. G. Recognition of antigenic differences among neurons using antiserums to clonal neural retina hybrid cells. Fed Proc. 1979 Sep;38(10):2368–2373. [PubMed] [Google Scholar]
  25. Yazulla S. Intraretinal differentiation in the synaptic organization of the inner plexiform layer of the pigeon retina. J Comp Neurol. 1974 Feb 1;153(3):309–324. doi: 10.1002/cne.901530307. [DOI] [PubMed] [Google Scholar]

Articles from Proceedings of the National Academy of Sciences of the United States of America are provided here courtesy of National Academy of Sciences

RESOURCES