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. 1981 Apr;78(4):2164–2168. doi: 10.1073/pnas.78.4.2164

Identification and localization of a gene that specifies production of Escherichia coli DNA topoisomerase I.

M Trucksis, R E Depew
PMCID: PMC319304  PMID: 6264465

Abstract

A gene that specifies production of Escherichia coli DNA topoisomerase I (omega protein) was identified with the aid of a radioimmunoassay for this protein. E. coli DNA topoisomerase I was produced by Salmonella typhimurium merodiploids that harbored E. coli plasmid F' 123, but not by strains that lost this plasmid. Analysis of strains with spontaneous deletions of F' 123 showed that the gene, topA, required for production of the E. coli omega protein was between the trp operon and the cysB gene. Deletions that eliminated topA also eliminated the supX gene. We suggest that topA is the structural gene of E. coli DNA topoisomerase I and that topA is identical to supX.

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Selected References

These references are in PubMed. This may not be the complete list of references from this article.

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