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. 1981 Apr;78(4):2218–2220. doi: 10.1073/pnas.78.4.2218

beta-Endorphin: characteristics of binding sites in a neuroblastoma--glioma hybrid cell.

R G Hammonds Jr, P Ferrara, C H Li
PMCID: PMC319315  PMID: 6264469

Abstract

Specific binding of human beta-endorphin to NG108-15 cells is described; human beta-[Tyr27-3H2] endorphin was used as the ligand. The binding is time dependent and saturable; Kd = 0.3 nM and ka = 1.8 x 10(8) M-1 min-1. Under the conditions optimal for beta-endorphin binding, leucine-enkephalin has one-fourth to one-third as many binding sites as beta-endorphin and its affinity is 7--10% that of beta-endorphin. Monovalent and divalent cations potently inhibit binding. Trypsin, phospholipase A, and N-ethylmaleimide reduce the ability of NG108-15 cells to bind beta-endorphin. beta-Endorphin analogs are able to fully inhibit the binding of beta-[Tyr27-3H2]endorphin, although enkephalins, morphine, and naloxone inhibit only 50--80%.

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Selected References

These references are in PubMed. This may not be the complete list of references from this article.

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