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. 1981 Apr;78(4):2320–2324. doi: 10.1073/pnas.78.4.2320

Host restriction of Friend leukemia virus: gag proteins of host range variants.

S Duttagupta, R Soeiro
PMCID: PMC319337  PMID: 6972535

Abstract

The host response to murine ecotropic leukemia viruses is mainly controlled by the mouse Fv-1 gene. This locus controls virus replication at an intracellular stage and prevents provirus integration. Biological studies suggest that the Fv-1 effector molecule recognizes at least one virion structural protein. We have produced host range variants of B-tropic Friend murine leukemia virus in order to study the primary structure of potential viral target proteins. Our results show that conversion of B-tropism to NB-tropism is associated with changes in the primary structure of three gag proteins--p15, p12, and p30. These results suggest that host range conversion is due to a recombinational event, presumably between the parental virus and an endogenous murine virus. They also open the possibility that p12 and p30 may be involved in host range restriction.

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Selected References

These references are in PubMed. This may not be the complete list of references from this article.

  1. Bassin R. H., Gerwin B. I., Duran-Troise G., Gisselbrecht S., Rein A. Murine sarcoma virus pseudotypes acquire a determinant specifying N or B tropism from leukaemia virus during rescue. Nature. 1975 Jul 17;256(5514):223–225. doi: 10.1038/256223a0. [DOI] [PubMed] [Google Scholar]
  2. Benjers B. M., Bassin R. H., Rein A., Gerwin B. I., Duran-Troise G. Mechanism of restriction of murine leukemia viruses varies between different strains of Fv-1n mice. Int J Cancer. 1979 Nov 15;24(5):600–607. doi: 10.1002/ijc.2910240513. [DOI] [PubMed] [Google Scholar]
  3. Elder J. H., Pickett R. A., 2nd, Hampton J., Lerner R. A. Radioiodination of proteins in single polyacrylamide gel slices. Tryptic peptide analysis of all the major members of complex multicomponent systems using microgram quantities of total protein. J Biol Chem. 1977 Sep 25;252(18):6510–6515. [PubMed] [Google Scholar]
  4. Faller D. V., Hopkins N. RNase T1-resistant oligonucleotides of B-tropic murine leukemia virus from BALB/c and five of its NB-tropic derivatives. J Virol. 1977 Jul;23(1):188–195. doi: 10.1128/jvi.23.1.188-195.1977. [DOI] [PMC free article] [PubMed] [Google Scholar]
  5. Gautsch J. W., Elder J. H., Schindler J., Jensen F. C., Lerner R. A. Structural markers on core protein p30 of murine leukemia virus: functional correlation with Fv-1 tropism. Proc Natl Acad Sci U S A. 1978 Sep;75(9):4170–4174. doi: 10.1073/pnas.75.9.4170. [DOI] [PMC free article] [PubMed] [Google Scholar]
  6. Huang A. S., Besmer P., Chu L., Baltimore D. Growth of pseudotypes of vesicular stomatitis virus with N-tropic murine leukemia virus coats in cells resistant to N-tropic viruses. J Virol. 1973 Sep;12(3):659–662. doi: 10.1128/jvi.12.3.659-662.1973. [DOI] [PMC free article] [PubMed] [Google Scholar]
  7. Ikeda H., Hardy W., Jr, Tress E., Fleissner E. Chromatographic separation and antigenic analysis of proteins of the oncornaviruses. V. Identification of a new murine viral protein, p15(E). J Virol. 1975 Jul;16(1):53–61. doi: 10.1128/jvi.16.1.53-61.1975. [DOI] [PMC free article] [PubMed] [Google Scholar]
  8. Jolicoeur P., Rassart E. Effect of Fv-1 gene product on synthesis of linear and supercoiled viral DNA in cells infected with murine leukemia virus. J Virol. 1980 Jan;33(1):183–195. doi: 10.1128/jvi.33.1.183-195.1980. [DOI] [PMC free article] [PubMed] [Google Scholar]
  9. Kashmiri S. V., Rein A., Bassin R. H., Gerwin B. I., Gisselbrecht S. Donation of N- or B-tropic phenotype to NB-tropic murine leukemia virus during mixed infections. J Virol. 1977 Jun;22(3):626–633. doi: 10.1128/jvi.22.3.626-633.1977. [DOI] [PMC free article] [PubMed] [Google Scholar]
  10. Laemmli U. K. Cleavage of structural proteins during the assembly of the head of bacteriophage T4. Nature. 1970 Aug 15;227(5259):680–685. doi: 10.1038/227680a0. [DOI] [PubMed] [Google Scholar]
  11. Lilly F., Steeves R. A. B-tropic Friend virus: a host-range pseudotype of spleen focus-forming virus (SFFV). Virology. 1973 Oct;55(2):363–370. doi: 10.1016/0042-6822(73)90176-1. [DOI] [PubMed] [Google Scholar]
  12. Lilly F. Susceptibility to two strains of Friend leukemia virus in mice. Science. 1967 Jan 27;155(3761):461–462. doi: 10.1126/science.155.3761.461. [DOI] [PubMed] [Google Scholar]
  13. Otten J. A., Myer F. E., Tennant R. W., Brown A. Effect of the Fv-1 locus in vivo: host range pseudotypes of murine sarcoma virus. J Natl Cancer Inst. 1978 Apr;60(4):875–880. doi: 10.1093/jnci/60.4.875. [DOI] [PubMed] [Google Scholar]
  14. Pincus T., Hartley J. W., Rowe W. P. A major genetic locus affecting resistance to infection with murine leukemia viruses. I. Tissue culture studies of naturally occurring viruses. J Exp Med. 1971 Jun 1;133(6):1219–1233. doi: 10.1084/jem.133.6.1219. [DOI] [PMC free article] [PubMed] [Google Scholar]
  15. Pincus T., Rowe W. P., Lilly F. A major genetic locus affecting resistance to infection with murine leukemia viruses. II. Apparent identity to a major locus described for resistance to friend murine leukemia virus. J Exp Med. 1971 Jun 1;133(6):1234–1241. doi: 10.1084/jem.133.6.1234. [DOI] [PMC free article] [PubMed] [Google Scholar]
  16. Rein A., Kashmiri S. V., Bassin R. H., Gerwin B. L., Duran-Troise G. Phenotypic mixing between N- and B-tropic murine leukemia viruses: infectious particles with dual sensitivity to Fv-1 restriction. Cell. 1976 Mar;7(3):373–379. doi: 10.1016/0092-8674(76)90166-5. [DOI] [PubMed] [Google Scholar]
  17. Rommelaere J., Donis-Keller H., Hopkins N. RNA sequencing provides evidence for allelism of determinants of the N-, B- or NB-tropism of murine leukemia viruses. Cell. 1979 Jan;16(1):43–50. doi: 10.1016/0092-8674(79)90186-7. [DOI] [PubMed] [Google Scholar]
  18. Schindler J., Hynes R., Hopkins N. Evidence for recombination between N- and B-tropic murine leukemia viruses: analysis of three virion proteins by sodium dodecyl sulfate-polyacrylamide gel electrophoresis. J Virol. 1977 Sep;23(3):700–700. doi: 10.1128/jvi.23.3.700-.1977. [DOI] [PMC free article] [PubMed] [Google Scholar]
  19. Sveda M. M., Soeiro R. Host restriction of Friend leukemia virus: synthesis and integration of the provirus. Proc Natl Acad Sci U S A. 1976 Jul;73(7):2356–2360. doi: 10.1073/pnas.73.7.2356. [DOI] [PMC free article] [PubMed] [Google Scholar]
  20. Yang W. K., Kiggans J. O., Yang D. M., Ou C. Y., Tennant R. W., Brown A., Bassin R. H. Synthesis and circularization of N- and B-tropic retroviral DNA Fv-1 permissive and restrictive mouse cells. Proc Natl Acad Sci U S A. 1980 May;77(5):2994–2998. doi: 10.1073/pnas.77.5.2994. [DOI] [PMC free article] [PubMed] [Google Scholar]

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