Abstract
We have isolated clonal cell lines from a nitrosomethylurea-induced mammary tumor in Wistar--Furth rats. The epithelial-like cells of these lines give rise to fibroblast-like cells. A study of several markers showed that the epithelial-like cells are similar to epithelial mammary cells. The fibroblastic cells, however, were similar to stromal cells, except for the production of collagen IV. The events occurring in these cultures are identical to those observed in previously isolated mammary cultures [Bennett, D. C., Peachey, L. A., Durbin, H. & Rudland, P. S. (1976) Cell 15, 283--298], suggesting their generality. The fibroblast-like cells generated in culture may be a hitherto unrecognized type of mammary cell.
Full text
PDF
Images in this article
Selected References
These references are in PubMed. This may not be the complete list of references from this article.
- Bennett D. C., Peachey L. A., Durbin H., Rudland P. S. A possible mammary stem cell line. Cell. 1978 Sep;15(1):283–298. doi: 10.1016/0092-8674(78)90104-6. [DOI] [PubMed] [Google Scholar]
- Bornstein P., Sage H. Structurally distinct collagen types. Annu Rev Biochem. 1980;49:957–1003. doi: 10.1146/annurev.bi.49.070180.004521. [DOI] [PubMed] [Google Scholar]
- Cohen L. A., Tsuang J., Chan P. C. Characteristics of rat normal mammary epithelial cells and dimethylbenzanthracene-induced mammary adenocarcinoma cells grown in monolayer culture. In Vitro. 1974 Jul-Aug;10:51–62. doi: 10.1007/BF02615338. [DOI] [PubMed] [Google Scholar]
- Dulbecco R., Bologna M., Unger M. Differentiation of a rat mammary cell line in vitro. Proc Natl Acad Sci U S A. 1979 Mar;76(3):1256–1260. doi: 10.1073/pnas.76.3.1256. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Franke W. W., Schmid E., Freudenstein C., Appelhans B., Osborn M., Weber K., Keenan T. W. Intermediate-sized filaments of the prekeratin type in myoepithelial cells. J Cell Biol. 1980 Mar;84(3):633–654. doi: 10.1083/jcb.84.3.633. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Franke W. W., Schmid E., Osborn M., Weber K. Intermediate-sized filaments of human endothelial cells. J Cell Biol. 1979 Jun;81(3):570–580. doi: 10.1083/jcb.81.3.570. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Gullino P. M., Pettigrew H. M., Grantham F. H. N-nitrosomethylurea as mammary gland carcinogen in rats. J Natl Cancer Inst. 1975 Feb;54(2):401–414. [PubMed] [Google Scholar]
- Hallowes R. C., Rudland P. S., Hawkins R. A., Lewis D. J., Bennet D., Durbin H. Comparison of the effects of hormones on DNA synthesis in cell cultures of nonneoplastic and neoplastic mammary epithelium from rats. Cancer Res. 1977 Aug;37(8 Pt 1):2492–2504. [PubMed] [Google Scholar]
- Lennon V. A., Unger M., Dulbecco R. Thy-1: a differentiation marker of potential mammary myoepithelial cells in vitro. Proc Natl Acad Sci U S A. 1978 Dec;75(12):6093–6097. doi: 10.1073/pnas.75.12.6093. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Listing F. Stardardized nomenclature for inbred strains of rats. Transplantation. 1973 Sep;16(3):221–245. [PubMed] [Google Scholar]
- Sun T. T., Green H. Immunofluorescent staining of keratin fibers in cultured cells. Cell. 1978 Jul;14(3):469–476. doi: 10.1016/0092-8674(78)90233-7. [DOI] [PubMed] [Google Scholar]