Abstract
Wounding (whether by excision, abrasion, or puncture) elicited rapid, massive, and enduring formation of polysomes in aged pea stems and other mature tissues. The response depended on temperature and severity of wounding but not on water uptake, and it occurred in tissues adjacent to, distant from, above, and below the site of injury. The kinetics of polysome formation were similar in tissues adjacent to or up to 150 mm distant from the point of injury. The wound-induced increases in protein-synthesizing capacity of the polysomes both in vivo and in vitro were much greater than the increases in rRNA and poly(A)+RNA. The results indicate that wounding evokes the almost immediate production of a wound signal that travels rapidly both acropetally and basipetally to stimulate the recruitment of preexisting ribosomes onto primarily preexisting mRNA, thence forming polysomes with greatly enhanced protein-synthesizing capacity.
Keywords: polyribosome formation, wheat germ system, poly(A) hybridization to poly(U), protein synthesis
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Selected References
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- Bishop J. O., Rosbash M. Polynucleotide sequences in eukaryotic DNA and RNA that form ribonuclease-resistant complexes with polyuridylic acid. J Mol Biol. 1974 May 5;85(1):75–86. doi: 10.1016/0022-2836(74)90130-2. [DOI] [PubMed] [Google Scholar]
- Bonner J., English J., Jr PURIFICATION OF TRAUMATIN, A PLANT WOUND HORMONE. Science. 1937 Oct 15;86(2233):352–353. doi: 10.1126/science.86.2233.352. [DOI] [PubMed] [Google Scholar]
- Davies E., Larkins B. A. Polyribosomes from Peas: II. Polyribosome Metabolism during Normal and Hormone-induced Growth. Plant Physiol. 1973 Oct;52(4):339–345. doi: 10.1104/pp.52.4.339. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Larkins B. A., Davies E. Polyribosomes from Peas: V. An Attempt to Characterize the Total Free and Membrane-bound Polysomal Population. Plant Physiol. 1975 Apr;55(4):749–756. doi: 10.1104/pp.55.4.749. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Nelson C. E., Ryan C. A. In vitro synthesis of pre-proteins of vacuolar compartmented proteinase inhibitors that accumulate in leaves of wounded tomato plants. Proc Natl Acad Sci U S A. 1980 Apr;77(4):1975–1979. doi: 10.1073/pnas.77.4.1975. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Roberts B. E., Paterson B. M. Efficient translation of tobacco mosaic virus RNA and rabbit globin 9S RNA in a cell-free system from commercial wheat germ. Proc Natl Acad Sci U S A. 1973 Aug;70(8):2330–2334. doi: 10.1073/pnas.70.8.2330. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Shen S. S., Steinhardt R. A. Intracellular pH controls the development of new potassium conductance after fertilization of the sea urchin egg. Exp Cell Res. 1980 Jan;125(1):55–61. doi: 10.1016/0014-4827(80)90188-3. [DOI] [PubMed] [Google Scholar]
- Travis R. L., Anderson J. M., Key J. L. Influence of auxin and incubation on the relative level of polyribosomes in excised soybean hypocotyl. Plant Physiol. 1973 Dec;52(6):608–612. doi: 10.1104/pp.52.6.608. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Walker D. J. Nursing 1980: new responsibility, new liability. Trial. 1980 Dec;16(12):43-7, 63. [PubMed] [Google Scholar]
- Wasilewska L. D., Cherry J. H. Polyribosome formation and RNA synthesis after breaking the dormancy of sugar beet root. Acta Biochim Pol. 1974;21(3):339–354. [PubMed] [Google Scholar]
- Yu Y. B., Yang S. F. Biosynthesis of wound ethylene. Plant Physiol. 1980 Aug;66(2):281–285. doi: 10.1104/pp.66.2.281. [DOI] [PMC free article] [PubMed] [Google Scholar]