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. 1981 Apr;78(4):2460–2464. doi: 10.1073/pnas.78.4.2460

Yeast genes fused to beta-galactosidase in Escherichia coli can be expressed normally in yeast.

M Rose, M J Casadaban, D Botstein
PMCID: PMC319366  PMID: 6787605

Abstract

A plasmid was constructed that allows the selection in vivo of gene fusions between the Escherichia coli beta-galactosidase gene and the yeast (Saccharomyces cerevisiae) URA3 gene. A large yeast DNA fragment containing the URA3 gene was placed upstream of an amino-terminally deleted version of the lacZ gene. The plasmid vehicle contains sequences that allow selection and maintenance of the plasmid in both yeast and E. coli. Selection for Lac+ in E. coli yielded numerous deletions that fused the lacZ gene to the URA3 gene and flanking yeast sequences, to the bacterial tetracycline-resistance gene from the parent plasmid pBR322, and to the yeast 2-micrometer plasmid DNA. Some of these fusion plasmids produced beta-galactosidase activity when introduced into yeast. One of the fusions to the URA3 gene itself has been shown to place the expression of beta-galactosidase activity under uracil regulation in yeasts.

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Selected References

These references are in PubMed. This may not be the complete list of references from this article.

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