Figure 7.

Natural killer T (NKT) cell-deficient mice show altered pattern of NK subsets. Splenocytes and lung mononuclear cells were isolated from wild-type (WT) and Jα18^−/− mice at day 3 following Chlamydia muridorum lung infection (2 × 10³ IFUs) and analysed by multi-colour flow cytometry. The gating strategy is depicted. (a–d) differential NK subsets in WT and Jα18^−/− mice. (a, c) Representative dot plots showing NK subsets in spleen (a) and lung (c) based on CD27 and CD11b expression on gated NK1.1⁺ CD3e⁻ cells. (b, d) Summary of the NK cell subsets in spleen (b) and lung (d). Data are mean ± SD of three mice in each group. Three independent experiments were performed and one representative experiment is shown. *P < 0·05.