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. 1981 May;78(5):3030–3033. doi: 10.1073/pnas.78.5.3030

Nature of the G1 phase of the yeast Saccharomyces cerevisiae.

R A Singer, G C Johnston
PMCID: PMC319493  PMID: 7019917

Abstract

Under conditions that protract the S phase for Saccharomyces cerevisiae without affecting steady-state rates of cell growth or proliferation, there were striking decreases in the length of the G1 period. These decreases were localized in the period between mitosis and the start event that initiates a new cell cycle. We conclude that this major fraction of the G1 period has no functional role in the DNA-division sequence of cell cycle events.

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Selected References

These references are in PubMed. This may not be the complete list of references from this article.

  1. Bücking-Throm E., Duntze W., Hartwell L. H., Manney T. R. Reversible arrest of haploid yeast cells in the initiation of DNA synthesis by a diffusible sex factor. Exp Cell Res. 1973 Jan;76(1):99–110. doi: 10.1016/0014-4827(73)90424-2. [DOI] [PubMed] [Google Scholar]
  2. Cooper S. A unifying model for the G1 period in prokaryotes and eukaryotes. Nature. 1979 Jul 5;280(5717):17–19. doi: 10.1038/280017a0. [DOI] [PubMed] [Google Scholar]
  3. Cooper S., Helmstetter C. E. Chromosome replication and the division cycle of Escherichia coli B/r. J Mol Biol. 1968 Feb 14;31(3):519–540. doi: 10.1016/0022-2836(68)90425-7. [DOI] [PubMed] [Google Scholar]
  4. Culotti J., Hartwell L. H. Genetic control of the cell division cycle in yeast. 3. Seven genes controlling nuclear division. Exp Cell Res. 1971 Aug;67(2):389–401. doi: 10.1016/0014-4827(71)90424-1. [DOI] [PubMed] [Google Scholar]
  5. Hartwell L. H., Culotti J., Pringle J. R., Reid B. J. Genetic control of the cell division cycle in yeast. Science. 1974 Jan 11;183(4120):46–51. doi: 10.1126/science.183.4120.46. [DOI] [PubMed] [Google Scholar]
  6. Hartwell L. H., Culotti J., Reid B. Genetic control of the cell-division cycle in yeast. I. Detection of mutants. Proc Natl Acad Sci U S A. 1970 Jun;66(2):352–359. doi: 10.1073/pnas.66.2.352. [DOI] [PMC free article] [PubMed] [Google Scholar]
  7. Hartwell L. H. Genetic control of the cell division cycle in yeast. II. Genes controlling DNA replication and its initiation. J Mol Biol. 1971 Jul 14;59(1):183–194. doi: 10.1016/0022-2836(71)90420-7. [DOI] [PubMed] [Google Scholar]
  8. Hartwell L. H. Macromolecule synthesis in temperature-sensitive mutants of yeast. J Bacteriol. 1967 May;93(5):1662–1670. doi: 10.1128/jb.93.5.1662-1670.1967. [DOI] [PMC free article] [PubMed] [Google Scholar]
  9. Hartwell L. H. Periodic density fluctuation during the yeast cell cycle and the selection of synchronous cultures. J Bacteriol. 1970 Dec;104(3):1280–1285. doi: 10.1128/jb.104.3.1280-1285.1970. [DOI] [PMC free article] [PubMed] [Google Scholar]
  10. Hartwell L. H. Saccharomyces cerevisiae cell cycle. Bacteriol Rev. 1974 Jun;38(2):164–198. doi: 10.1128/br.38.2.164-198.1974. [DOI] [PMC free article] [PubMed] [Google Scholar]
  11. Hartwell L. H. Three additional genes required for deoxyribonucleic acid synthesis in Saccharomyces cerevisiae. J Bacteriol. 1973 Sep;115(3):966–974. doi: 10.1128/jb.115.3.966-974.1973. [DOI] [PMC free article] [PubMed] [Google Scholar]
  12. Hartwell L. H., Unger M. W. Unequal division in Saccharomyces cerevisiae and its implications for the control of cell division. J Cell Biol. 1977 Nov;75(2 Pt 1):422–435. doi: 10.1083/jcb.75.2.422. [DOI] [PMC free article] [PubMed] [Google Scholar]
  13. Hereford L. M., Hartwell L. H. Sequential gene function in the initiation of Saccharomyces cerevisiae DNA synthesis. J Mol Biol. 1974 Apr 15;84(3):445–461. doi: 10.1016/0022-2836(74)90451-3. [DOI] [PubMed] [Google Scholar]
  14. Jaenicke L., Scholz K., Donike M. Synthese der Dihydrofolat-Reduktase in synchronisierter Hefe. Zusammenhang zwischen DNA-Replikation und stufenförmigem Syntheseverlau. Eur J Biochem. 1970 Mar 1;13(1):137–141. doi: 10.1111/j.1432-1033.1970.tb00909.x. [DOI] [PubMed] [Google Scholar]
  15. Jagadish M. N., Carter B. L. Genetic control of cell division in yeast cultured at different growth rates. Nature. 1977 Sep 8;269(5624):145–147. doi: 10.1038/269145a0. [DOI] [PubMed] [Google Scholar]
  16. Johnston G. C., Ehrhardt C. W., Lorincz A., Carter B. L. Regulation of cell size in the yeast Saccharomyces cerevisiae. J Bacteriol. 1979 Jan;137(1):1–5. doi: 10.1128/jb.137.1.1-5.1979. [DOI] [PMC free article] [PubMed] [Google Scholar]
  17. Johnston G. C., Pringle J. R., Hartwell L. H. Coordination of growth with cell division in the yeast Saccharomyces cerevisiae. Exp Cell Res. 1977 Mar 1;105(1):79–98. doi: 10.1016/0014-4827(77)90154-9. [DOI] [PubMed] [Google Scholar]
  18. Johnston G. C., Singer R. A. RNA synthesis and control of cell division in the yeast S. cerevisiae. Cell. 1978 Aug;14(4):951–958. doi: 10.1016/0092-8674(78)90349-5. [DOI] [PubMed] [Google Scholar]
  19. Liskay R. M. Genetic analysis of a Chinese hamster cell line lacking a G1 phase. Exp Cell Res. 1978 Jun;114(1):69–77. doi: 10.1016/0014-4827(78)90037-x. [DOI] [PubMed] [Google Scholar]
  20. Liskay R. M., Kornfeld B., Fullerton P., Evans R. Protein synthesis and the presence of absence of a measurable G1 in cultured Chinese hamster cells. J Cell Physiol. 1980 Sep;104(3):461–467. doi: 10.1002/jcp.1041040318. [DOI] [PubMed] [Google Scholar]
  21. Liskay R. M., Leonard K. E., Prescott D. M. Different Chinese hamster cell lines express a G1 period for different reasons. Somatic Cell Genet. 1979 Sep;5(5):615–623. doi: 10.1007/BF01542698. [DOI] [PubMed] [Google Scholar]
  22. Liskay R. M., Prescott D. M. Genetic analysis of the G1 period: isolation of mutants (or variants) with a G1 perior from a Chinese hamster cell line lacking G1. Proc Natl Acad Sci U S A. 1978 Jun;75(6):2873–2877. doi: 10.1073/pnas.75.6.2873. [DOI] [PMC free article] [PubMed] [Google Scholar]
  23. Pardee A. B., Dubrow R., Hamlin J. L., Kletzien R. F. Animal cell cycle. Annu Rev Biochem. 1978;47:715–750. doi: 10.1146/annurev.bi.47.070178.003435. [DOI] [PubMed] [Google Scholar]
  24. Pringle J. R., Mor J. R. Methods for monitoring the growth of yeast cultures and for dealing with the clumping problem. Methods Cell Biol. 1975;11:131–168. doi: 10.1016/s0091-679x(08)60320-9. [DOI] [PubMed] [Google Scholar]
  25. Pringle J. R. The use of conditional lethal cell cycle mutants for temporal and functional sequence mapping of cell cycle events. J Cell Physiol. 1978 Jun;95(3):393–405. doi: 10.1002/jcp.1040950318. [DOI] [PubMed] [Google Scholar]
  26. Pritchard R. H., Zaritsky A. Effect of thymine concentration on the replication velocity of DNA in a thymineless mutant of Escherichia coli. Nature. 1970 Apr 11;226(5241):126–131. doi: 10.1038/226126a0. [DOI] [PubMed] [Google Scholar]
  27. Robinow C. F. The preparation of yeasts for light microscopy. Methods Cell Biol. 1975;11:1–22. doi: 10.1016/s0091-679x(08)60314-3. [DOI] [PubMed] [Google Scholar]
  28. Slater M. L. Effect of reversible inhibition of deoxyribonucleic acid synthesis on the yeast cell cycle. J Bacteriol. 1973 Jan;113(1):263–270. doi: 10.1128/jb.113.1.263-270.1973. [DOI] [PMC free article] [PubMed] [Google Scholar]
  29. Slater M. L., Sharrow S. O., Gart J. J. Cell cycle of Saccharomycescerevisiae in populations growing at different rates. Proc Natl Acad Sci U S A. 1977 Sep;74(9):3850–3854. doi: 10.1073/pnas.74.9.3850. [DOI] [PMC free article] [PubMed] [Google Scholar]
  30. Unger M. W., Hartwell L. H. Control of cell division in Saccharomyces cerevisiae by methionyl-tRNA. Proc Natl Acad Sci U S A. 1976 May;73(5):1664–1668. doi: 10.1073/pnas.73.5.1664. [DOI] [PMC free article] [PubMed] [Google Scholar]

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