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Proceedings of the National Academy of Sciences of the United States of America logoLink to Proceedings of the National Academy of Sciences of the United States of America
. 1981 Jun;78(6):3388–3392. doi: 10.1073/pnas.78.6.3388

Distribution of UV light-induced damage in a defined sequence of human DNA: detection of alkaline-sensitive lesions at pyrimidine nucleoside-cytidine sequences.

J A Lippke, L K Gordon, D E Brash, W A Haseltine
PMCID: PMC319573  PMID: 6943547

Abstract

The distribution of UV light-induced damage to the highly reiterated alpha sequence of human DNA was investigated. The results show that the distribution of UV light-induced cyclobutane dimers within a defined sequence is similar whether the DNA is exposed to UV light as part of the chromosome of intact cells or as naked DNA. However, the cellular environment shields the nuclear DNA, resulting in about 50% decrease in apparent dose. A new type of UV photodamage was detected. Treatment of UV light-irradiated DNA with hot alkali results in strand breaks at positions of cytidine located 3' to pyrimidine nucleosides. The chemical nature and biological significance of the pyrimidine nucleoside-cytidine lesion is discussed.

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Selected References

These references are in PubMed. This may not be the complete list of references from this article.

  1. Calos M. P., Johnsrud L., Miller J. H. DNA sequence at the integration sites of the insertion element IS1. Cell. 1978 Mar;13(3):411–418. doi: 10.1016/0092-8674(78)90315-x. [DOI] [PubMed] [Google Scholar]
  2. Coulondre C., Miller J. H., Farabaugh P. J., Gilbert W. Molecular basis of base substitution hotspots in Escherichia coli. Nature. 1978 Aug 24;274(5673):775–780. doi: 10.1038/274775a0. [DOI] [PubMed] [Google Scholar]
  3. D'Andrea A. D., Haseltine W. A. Sequence specific cleavage of DNA by the antitumor antibiotics neocarzinostatin and bleomycin. Proc Natl Acad Sci U S A. 1978 Aug;75(8):3608–3612. doi: 10.1073/pnas.75.8.3608. [DOI] [PMC free article] [PubMed] [Google Scholar]
  4. Farabaugh P. J. Sequence of the lacI gene. Nature. 1978 Aug 24;274(5673):765–769. doi: 10.1038/274765a0. [DOI] [PubMed] [Google Scholar]
  5. Gordon L. K., Haseltine W. A. Comparison of the cleavage of pyrimidine dimers by the bacteriophage T4 and Micrococcus luteus UV-specific endonucleases. J Biol Chem. 1980 Dec 25;255(24):12047–12050. [PubMed] [Google Scholar]
  6. Grunberg S. M., Haseltine W. A. Use of an indicator sequence of human DNA to study DNA damage by methylbis(2-chloroethyl)amine. Proc Natl Acad Sci U S A. 1980 Nov;77(11):6546–6550. doi: 10.1073/pnas.77.11.6546. [DOI] [PMC free article] [PubMed] [Google Scholar]
  7. Hanawalt P. C., Cooper P. K., Ganesan A. K., Smith C. A. DNA repair in bacteria and mammalian cells. Annu Rev Biochem. 1979;48:783–836. doi: 10.1146/annurev.bi.48.070179.004031. [DOI] [PubMed] [Google Scholar]
  8. Haseltine W. A., Gordon L. K., Lindan C. P., Grafstrom R. H., Shaper N. L., Grossman L. Cleavage of pyrimidine dimers in specific DNA sequences by a pyrimidine dimer DNA-glycosylase of M. luteus. Nature. 1980 Jun 26;285(5767):634–641. doi: 10.1038/285634a0. [DOI] [PubMed] [Google Scholar]
  9. Haseltine W. A., Lindan C. P., D'Andrea A. D., Johnsrud L. The use of DNA fragments of defined sequence for the study of DNA damage and repair. Methods Enzymol. 1980;65(1):235–248. doi: 10.1016/s0076-6879(80)65033-2. [DOI] [PubMed] [Google Scholar]
  10. Johnsrud L. Contacts between Escherichia coli RNA polymerase and a lac operon promoter. Proc Natl Acad Sci U S A. 1978 Nov;75(11):5314–5318. doi: 10.1073/pnas.75.11.5314. [DOI] [PMC free article] [PubMed] [Google Scholar]
  11. Maio J. J., Brown F. L., Musich P. R. Subunit structure of chromatin and the organization of eukaryotic highly repetitive DNA: recurrent periodicities and models for the evolutionary origins of repetitive DNA. J Mol Biol. 1977 Dec 15;117(3):637–655. doi: 10.1016/0022-2836(77)90062-6. [DOI] [PubMed] [Google Scholar]
  12. Manuelidis L. Repeating restriction fragments of human DNA. Nucleic Acids Res. 1976 Nov;3(11):3063–3076. doi: 10.1093/nar/3.11.3063. [DOI] [PMC free article] [PubMed] [Google Scholar]
  13. Manuelidis L., Wu J. C. Homology between human and simian repeated DNA. Nature. 1978 Nov 2;276(5683):92–94. doi: 10.1038/276092a0. [DOI] [PubMed] [Google Scholar]
  14. Maxam A. M., Gilbert W. A new method for sequencing DNA. Proc Natl Acad Sci U S A. 1977 Feb;74(2):560–564. doi: 10.1073/pnas.74.2.560. [DOI] [PMC free article] [PubMed] [Google Scholar]
  15. Radman M. An endonuclease from Escherichia coli that introduces single polynucleotide chain scissions in ultraviolet-irradiated DNA. J Biol Chem. 1976 Mar 10;251(5):1438–1445. [PubMed] [Google Scholar]
  16. Riazuddin S., Grossman L. Micrococcus luteus correndonucleases. I. resolution and purification of two endonucleases specific for DNA containing pyrimidine dimers. J Biol Chem. 1977 Sep 25;252(18):6280–6286. [PubMed] [Google Scholar]
  17. Shaper N. L., Grossman L. Purification and properties of the human placental apurinic/apyrimidinic endonuclease. Methods Enzymol. 1980;65(1):216–224. doi: 10.1016/s0076-6879(80)65030-7. [DOI] [PubMed] [Google Scholar]
  18. Varghese A. J. Photochemical reactions of cytosine nucleosides in frozen aqueous solution in deoxyribonucleic acid. Biochemistry. 1971 Jun 8;10(12):2194–2199. doi: 10.1021/bi00788a003. [DOI] [PubMed] [Google Scholar]
  19. Varghese A. J., Wang S. Y. Ultraviolet irradiation of DNA in vitro and in vivo produces a 3d thymine-derived product. Science. 1967 May 19;156(3777):955–957. doi: 10.1126/science.156.3777.955. [DOI] [PubMed] [Google Scholar]
  20. Wang S. Y., Varghese A. J. Cytosine-thymine addition product from DNA irradiated with ultraviolet light. Biochem Biophys Res Commun. 1967 Nov 30;29(4):543–549. doi: 10.1016/0006-291x(67)90519-0. [DOI] [PubMed] [Google Scholar]
  21. Wu J. C., Manuelidis L. Sequence definition and organization of a human repeated DNA. J Mol Biol. 1980 Sep 25;142(3):363–386. doi: 10.1016/0022-2836(80)90277-6. [DOI] [PubMed] [Google Scholar]

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