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. 1981 Jun;78(6):3469–3472. doi: 10.1073/pnas.78.6.3469

Partial characterization of gamma (immune) interferon mRNA extracted from human lymphocytes.

T Taniguchi, R H Pang, Y K Yip, D Henriksen, J Vilcek
PMCID: PMC319590  PMID: 6167986

Abstract

gamma (immune) interferon (IFN-gamma) was induced in cultures of fresh human lymphocytes by combined treatment with a phorbol ester (12-O-tetradecanoylphorbol 13-acetate, TPA) and the T cell mitogen phytohemagglutinin (PHA). Compared to the IFN-gamma yields obtained with PHA induction alone, the inclusion of TPA caused a significant enhancement of IFN-gamma production. Poly(A)-containing mRNA was isolated from mononuclear cells induced with TPA and PHA. Injected into Xenopus laevis oocytes, this mRNA preparation gave rise to IFN activity with characteristic properties of human IFN-gamma. Sucrose density gradient centrifugation analysis showed that IFN-gamma mRNA sedimented at 15 S, suggesting that it contains a total of about 1400 nucleotides.

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Selected References

These references are in PubMed. This may not be the complete list of references from this article.

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