Skip to main content
Proceedings of the National Academy of Sciences of the United States of America logoLink to Proceedings of the National Academy of Sciences of the United States of America
. 1981 Jun;78(6):3536–3540. doi: 10.1073/pnas.78.6.3536

Proteinase inhibitor-inducing factor activity in tomato leaves resides in oligosaccharides enzymically released from cell walls

Paul D Bishop 1, Donald J Makus 1, Gregory Pearce 1, Clarence A Ryan 1,*
PMCID: PMC319604  PMID: 16593033

Abstract

The synthesis and accumulation of proteinase inhibitor I in excised tomato leaves can be induced with oligosaccharides obtained by fungal endo-α-1,4-polygalacturonase digestion of a pectic polysaccharide (Mr 5000-10,000) isolated from tomato leaves. Active oligosaccharides were also released from isolated tomato leaf cell walls by endopolygalacturonases partially purified from tomato plants. It is suggested that oligosaccharides, released from plant cell wall pectic polysaccharides by either endogenous or exogenous endopolygalacturonases at a wound or infection site, may have hormone-like roles in regulating plant defense responses in unwounded tissues many centimeters away from the site of release.

Keywords: endopolygalacturonases, pectic fragments, wound hormone, pest attack

Full text

PDF
3536

Selected References

These references are in PubMed. This may not be the complete list of references from this article.

  1. Lee M., Macmillan J. D. Mode of action of pectic enzymes. I. Purification and certain properties of tomato pectinesterase. Biochemistry. 1968 Nov;7(11):4005–4010. doi: 10.1021/bi00851a030. [DOI] [PubMed] [Google Scholar]
  2. Lee S. C., West C. A. Polygalacturonase from Rhizopus stolonifer, an Elicitor of Casbene Synthetase Activity in Castor Bean (Ricinus communis L.) Seedlings. Plant Physiol. 1981 Apr;67(4):633–639. doi: 10.1104/pp.67.4.633. [DOI] [PMC free article] [PubMed] [Google Scholar]
  3. McNeil M., Darvill A. G., Albersheim P. Structure of Plant Cell Walls: X. RHAMNOGALACTURONAN I, A STRUCTURALLY COMPLEX PECTIC POLYSACCHARIDE IN THE WALLS OF SUSPENSION-CULTURED SYCAMORE CELLS. Plant Physiol. 1980 Dec;66(6):1128–1134. doi: 10.1104/pp.66.6.1128. [DOI] [PMC free article] [PubMed] [Google Scholar]
  4. Pressey R., Avants J. K. Two forms of polygalacturonase in tomatoes. Biochim Biophys Acta. 1973 Jun 6;309(2):363–369. doi: 10.1016/0005-2744(73)90035-1. [DOI] [PubMed] [Google Scholar]
  5. Ryan C. A. The regulation by carbon dioxide of protein synthesis in tomato leaves. Biochem Biophys Res Commun. 1977 Aug 8;77(3):1004–1008. doi: 10.1016/s0006-291x(77)80077-6. [DOI] [PubMed] [Google Scholar]
  6. Ryan C. A. Wound-regulated synthesis and vacuolar compartmentation of proteinase inhibitors in plant leaves. Curr Top Cell Regul. 1980;17:1–23. doi: 10.1016/b978-0-12-152817-1.50005-5. [DOI] [PubMed] [Google Scholar]
  7. SMOGYI M. Notes on sugar determination. J Biol Chem. 1952 Mar;195(1):19–23. [PubMed] [Google Scholar]

Articles from Proceedings of the National Academy of Sciences of the United States of America are provided here courtesy of National Academy of Sciences

RESOURCES