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. Author manuscript; available in PMC: 2012 Jun 19.
Published in final edited form as: Hippocampus. 2011 Apr 20;22(3):590–603. doi: 10.1002/hipo.20923

FIGURE 1.

FIGURE 1

Influence of post mortem time on GAD67-IR and mRNA levels in the rat brain. Rats were killed and decapitated. Their sculls were kept for 0, 8, and 24 h at 16°C. Their brains were then removed and either immersion fixed with paraformaldehyde for subsequent immunohistochemistry or snap frozen for in situ hybridization. In A, D, and G GAD67-IR is shown in the dentate gyrus at the post mortem intervals of 0, 8, and 24 h, respectively. In B, E, H, GAD67-IR is shown in the prefrontal cortex region revealing survival of GAD67-IR neurons at the same intervals. In C, F, I GAD67 mRNA is shown at post mortem intervals of 0, 8, and 24 h. In J and K relative optical density (ROD) values are shown for the mossy fibers in the tip of the hilus (A, D, G) and for the granule cell layer (C, F, I), respectively. The numbers of animals per group were 4. Statistical analysis was done by ANOVA with Bonferoni post hoc test. P refers to the nonexposed (0 h) group.