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. Author manuscript; available in PMC: 2012 Jun 19.
Published in final edited form as: Hippocampus. 2011 Apr 20;22(3):590–603. doi: 10.1002/hipo.20923

FIGURE 4.

FIGURE 4

Double immunofluorescence for GAD67 and the mossy fiber marker dynorphin in terminal areas of mossy fibers (A–I) and of GAD67 and VGAT in GABAergic fibers of the dentate gyrus (J–L) in sections from patients with hippocampal sclerosis. A–C shows colabeling of the stratum lucidum CA3 with GAD67- and dynorphin-IR (patient E18); D–F depicts colabeling of mossy fiber bundles in the dentate hilus (h) with GAD67-IR and dynorphin-IR (patient E18). In G–I labeling of the dentate molecular layer of a sclerotic specimen is shown for GAD67- and dynorphin-IR (case E41). Note colabeling of GAD67 with the mossy fiber marker dynorphin in the inner but not outer molecular layer (this is the terminal area of sprouted mossy fibers in hippocampal sclerosis). On the other hand, VGAT a marker of GABA-ergic neurons labels the outer molecular layer (K, L). Labeling for GAD67-IR again is strong in the inner (J, L) and faint in the outer (J) molecular layer. Scale bars: in C for A–C: 200 μm; in F for D–F: 50 μm, in I and L for G–L: 200 μm.