Abstract
Three cell surface antigens that are structurally related to the human major histocompatibility antigens (called HLA antigens) have been characterized from the leukemic T cell line MOLT-4. One antigen is a glycoprotein of Mr 49,000 recognized by two monoclonal antibodies. OKT6 and NA1/34, and is associated with a Mr 12,000 subunit that crossreacts serologically with beta 2-microglobulin but can be distinguished from it by sodium dodecyl sulfate/polyacrylamide gel electrophoresis. A second antigen, defined by the monoclonal antibody OKT10, is a Mr 46,000 protein associated with a small subunit distinct from beta 2-microglobulin. The OKT10 antigen is not restricted to T cells and is found on all T and B lymphoblastoid cell lines tested. The third protein is a beta 2-microglobulin-associated glycoprotein of Mr 43,000 that is serologically distinct from the OKT6 (NA1/34), OKT10, and HLA antigens. It is found on some, but not all, T cell lines but is absent from any other hematopoietic cell lines tested.
Full text
PDF![3858](https://cdn.ncbi.nlm.nih.gov/pmc/blobs/7d96/319672/3b71c4e7eb32/pnas00657-0596.png)
![3859](https://cdn.ncbi.nlm.nih.gov/pmc/blobs/7d96/319672/a7d2000a38f2/pnas00657-0597.png)
![3860](https://cdn.ncbi.nlm.nih.gov/pmc/blobs/7d96/319672/880b431a0968/pnas00657-0598.png)
![3861](https://cdn.ncbi.nlm.nih.gov/pmc/blobs/7d96/319672/7c2f46b1fb6b/pnas00657-0599.png)
![3862](https://cdn.ncbi.nlm.nih.gov/pmc/blobs/7d96/319672/a34a5ded22c9/pnas00657-0600.png)
Images in this article
Selected References
These references are in PubMed. This may not be the complete list of references from this article.
- Barnstable C. J., Bodmer W. F., Brown G., Galfre G., Milstein C., Williams A. F., Ziegler A. Production of monoclonal antibodies to group A erythrocytes, HLA and other human cell surface antigens-new tools for genetic analysis. Cell. 1978 May;14(1):9–20. doi: 10.1016/0092-8674(78)90296-9. [DOI] [PubMed] [Google Scholar]
- Brodsky F. M., Bodmer W. F., Parham P. Characterization of a monoclonal anti-beta 2-microglobulin antibody and its use in the genetic and biochemical analysis of major histocompatibility antigens. Eur J Immunol. 1979 Jul;9(7):536–545. doi: 10.1002/eji.1830090709. [DOI] [PubMed] [Google Scholar]
- Fithian E., Kung P., Goldstein G., Rubenfeld M., Fenoglio C., Edelson R. Reactivity of Langerhans cells with hybridoma antibody. Proc Natl Acad Sci U S A. 1981 Apr;78(4):2541–2544. doi: 10.1073/pnas.78.4.2541. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Hansen T. H., Cullen S. E., Sachs D. H. Immunochemical evidence for an additional H-2 region closely linked to H-2D. J Exp Med. 1977 Feb 1;145(2):438–442. doi: 10.1084/jem.145.2.438. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Hämmerling G. J., Hämmerling U., Flaherty L. Qat-4 and Qat-5, new murine T-cell antigens governed by the Tla region and identified by monoclonal antibodies. J Exp Med. 1979 Jul 1;150(1):108–116. doi: 10.1084/jem.150.1.108. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Janossy G., Tidman N., Selby W. S., Thomas J. A., Granger S., Kung P. C., Goldstein G. Human T lymphocytes of inducer and suppressor type occupy different microenvironments. Nature. 1980 Nov 6;288(5786):81–84. doi: 10.1038/288081a0. [DOI] [PubMed] [Google Scholar]
- Jongsma A., van Someren H., Westerveld A., Hagemeijer A., Pearson P. Localization of genes on human chromosomes by studies of human-Chinese hamster somatic cell hybrids. Assignment of PGM3 to chromosome C6 and regional mapping of the PGD, PGM1 and pep-C genes on chromosome A1. Humangenetik. 1973 Dec 10;20(3):195–202. doi: 10.1007/BF00385730. [DOI] [PubMed] [Google Scholar]
- Judd W., Poodry C. A., Broder S., Friedman S. M., Chess L., Strominger J. L. High molecular weight antigens present on human T cells. Proc Natl Acad Sci U S A. 1980 Nov;77(11):6805–6809. doi: 10.1073/pnas.77.11.6805. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Kincade P. W., Flaherty L., Lee G., Watanabe T., Michaelson J. Qa antigen expression on functional lymphoid, myeloid, and stem cells in adult mice. J Immunol. 1980 Jun;124(6):2879–2885. [PubMed] [Google Scholar]
- Krangel M. S., Orr H. T., Strominger J. L. Assembly and maturation of HLA-A and HLA-B antigens in vivo. Cell. 1979 Dec;18(4):979–991. doi: 10.1016/0092-8674(79)90210-1. [DOI] [PubMed] [Google Scholar]
- Laemmli U. K. Cleavage of structural proteins during the assembly of the head of bacteriophage T4. Nature. 1970 Aug 15;227(5259):680–685. doi: 10.1038/227680a0. [DOI] [PubMed] [Google Scholar]
- Markwell M. A., Fox C. F. Surface-specific iodination of membrane proteins of viruses and eucaryotic cells using 1,3,4,6-tetrachloro-3alpha,6alpha-diphenylglycoluril. Biochemistry. 1978 Oct 31;17(22):4807–4817. doi: 10.1021/bi00615a031. [DOI] [PubMed] [Google Scholar]
- McMichael A. J., Pilch J. R., Galfré G., Mason D. Y., Fabre J. W., Milstein C. A human thymocyte antigen defined by a hybrid myeloma monoclonal antibody. Eur J Immunol. 1979 Mar;9(3):205–210. doi: 10.1002/eji.1830090307. [DOI] [PubMed] [Google Scholar]
- Michaelson J., Flaherty L., Vitetta E., Poulik M. D. Molecular similarities between the Qa-2 alloantigen and other gene products of the 17th chromosome of the mouse. J Exp Med. 1977 Apr 1;145(4):1066–1070. doi: 10.1084/jem.145.4.1066. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Michaelson J., Rothenberg E., Boyse E. A. Genetic polymorphism of murine beta 2-microglobulin detected biochemically. Immunogenetics. 1980 Jul;11(1):93–95. doi: 10.1007/BF01567773. [DOI] [PubMed] [Google Scholar]
- Parham P., Barnstable C. J., Bodmer W. F. Use of a monoclonal antibody (W6/32) in structural studies of HLA-A,B,C, antigens. J Immunol. 1979 Jul;123(1):342–349. [PubMed] [Google Scholar]
- Ploegh H. L., Cannon L. E., Strominger J. L. Cell-free translation of the mRNAs for the heavy and light chains of HLA-A and HLA-B antigens. Proc Natl Acad Sci U S A. 1979 May;76(5):2273–2277. doi: 10.1073/pnas.76.5.2273. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Reinherz E. L., Kung P. C., Goldstein G., Levey R. H., Schlossman S. F. Discrete stages of human intrathymic differentiation: analysis of normal thymocytes and leukemic lymphoblasts of T-cell lineage. Proc Natl Acad Sci U S A. 1980 Mar;77(3):1588–1592. doi: 10.1073/pnas.77.3.1588. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Reinherz E. L., Kung P. C., Goldstein G., Schlossman S. F. A monoclonal antibody with selective reactivity with functionally mature human thymocytes and all peripheral human T cells. J Immunol. 1979 Sep;123(3):1312–1317. [PubMed] [Google Scholar]
- Rothenberg E., Boyse E. A. Synthesis and processing of molecules bearing thymus leukemia antigen. J Exp Med. 1979 Oct 1;150(4):777–791. doi: 10.1084/jem.150.4.777. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Sonoda S., Schlamowitz M. Studies of 125I trace labeling of immunoglobulin G by chloramine-T. Immunochemistry. 1970 Nov;7(11):885–898. doi: 10.1016/0019-2791(70)90051-0. [DOI] [PubMed] [Google Scholar]
- Stanton T. H., Hood L. Biochemical identification of the Qa-1 alloantigen. Immunogenetics. 1980;11(3):309–314. doi: 10.1007/BF01567797. [DOI] [PubMed] [Google Scholar]
- Tada N., Tanigaki N., Pressman D. Human cell membrane components bound to beta2-microglobulin in T cell-type cell lines. J Immunol. 1978 Feb;120(2):513–519. [PubMed] [Google Scholar]
- Tanigaki N., Tokuyama H., Fukunishi T., Minowada J., Pressman D. Human cell membrane components dominant in T cell lineage: identification and characterization of human TL-like antigens. J Immunol. 1979 Dec;123(6):2906–2914. [PubMed] [Google Scholar]
- Vitetta E. S., Uhr J. W., Boyse E. A. Association of a beta2-microglobulin-like subunit with H-2 and TL alloantigens on murine thymocytes. J Immunol. 1975 Jan;114(1 Pt 1):252–254. [PubMed] [Google Scholar]
- Ziegler A., Milstein C. A small polypeptide different from beta2-microglobin associated with a human cell surface antigen. Nature. 1979 May 17;279(5710):243–244. doi: 10.1038/279243a0. [DOI] [PubMed] [Google Scholar]